TY  - JOUR
A1  - Wille, Michael
A1  - Schümann, Antje
A1  - Kreutzer, Michael
A1  - Glocker, Michael O
A1  - Wree, Andreas
A1  - Mutzbauer, Grit
A1  - Schmitt, Oliver
T1  - The proteome profiles of the olfactory bulb of juvenile, adult and aged rats - an ontogenetic study
JF  - Proteome Science
N2  - Background: 
In this study, we searched for proteins that change their expression in the olfactory bulb (oB) of rats during ontogenesis. Up to now, protein expression differences in the developing animal are not fully understood. Our investigation focused on the question whether specific proteins exist which are only expressed during different development stages. This might lead to a better characterization of the microenvironment and to a better determination of factors and candidates that influence the differentiation of neuronal progenitor cells. 

Results: 
After analyzing the samples by two-dimensional polyacrylamide gel electrophoresis (2DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), it could be shown that the number of expressed proteins differs depending on the developmental stages. Especially members of the functional classes, like proteins of biosynthesis, regulatory proteins and structural proteins, show the highest differential expression in the stages of development analyzed. 

Conclusion: 
In this study, quantitative changes in the expression of proteins in the oB at different developmental stages (postnatal days (P) 7, 90 and 637) could be observed. Furthermore, the expression of many proteins was found at specific developmental stages. It was possible to identify these proteins which are involved in processes like support of cell migration and differentiation.
KW  - axonally transported proteins
KW  - hippocampal stem cells
KW  - cerebral cortex
KW  - regional development
KW  - development
KW  - brain
KW  - olfactory bulb
KW  - proteomics
KW  - rat
KW  - growth-associated protein
KW  - messenger-RNA transport
KW  - goldfish optic nerve
KW  - postnatal development
KW  - subventricular zone
KW  - neuronal differentiation
Y1  - 2015
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-144073
VL  - 13
IS  - 8
ER  -