TY  - JOUR
A1  - Wörsdörfer, Philipp
A1  - Dalda, Nahide
A1  - Kern, Anna
A1  - Krüger, Sarah
A1  - Wagner, Nicole
A1  - Kwok, Chee Keong
A1  - Henke, Erik
A1  - Ergün, Süleyman
T1  - Generation of complex human organoid models including vascular networks by incorporation of mesodermal progenitor cells
JF  - Scientific Reports
N2  - Organoids derived from human pluripotent stem cells are interesting models to study mechanisms of morphogenesis and promising platforms for disease modeling and drug screening. However, they mostly remain incomplete as they lack stroma, tissue resident immune cells and in particular vasculature, which create important niches during development and disease. We propose, that the directed incorporation of mesodermal progenitor cells (MPCs) into organoids will overcome the aforementioned limitations. In order to demonstrate the feasibility of the method, we generated complex human tumor as well as neural organoids. We show that the formed blood vessels display a hierarchic organization and mural cells are assembled into the vessel wall. Moreover, we demonstrate a typical blood vessel ultrastructure including endothelial cell-cell junctions, a basement membrane as well as luminal caveolae and microvesicles. We observe a high plasticity in the endothelial network, which expands, while the organoids grow and is responsive to anti-angiogenic compounds and pro-angiogenic conditions such as hypoxia. We show that vessels within tumor organoids connect to host vessels following transplantation. Remarkably, MPCs also deliver Iba1\(^+\) cells that infiltrate the neural tissue in a microglia-like manner.
KW  - Developmental biology
KW  - Stem cells
Y1  - 2019
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-202681
VL  - 9
ER  - 
TY  - JOUR
A1  - Wörsdörfer, Philipp
A1  - Ergün, Süleyman
T1  - “Organoids”: insights from the first issues
JF  - Organoids
N2  - No abstract available
KW  - organoids
KW  - editorial
Y1  - 2023
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-313694
SN  - 2674-1172
VL  - 2
IS  - 2
SP  - 79
EP  - 81
ER  - 
TY  - JOUR
A1  - Wörsdörfer, Philipp
A1  - I, Takashi
A1  - Asahina, Izumi
A1  - Sumita, Yoshinori
A1  - Ergün, Süleyman
T1  - Do not keep it simple: recent advances in the generation of complex organoids
JF  - Journal of Neural Transmission
N2  - 3D cell culture models which closely resemble real human tissues are of high interest for disease modelling, drug screening as well as a deeper understanding of human developmental biology. Such structures are termed organoids. Within the last years, several human organoid models were described. These are usually stem cell derived, arise by self-organization, mimic mechanisms of normal tissue development, show typical organ morphogenesis and recapitulate at least some organ specific functions. Many tissues have been reproduced in vitro such as gut, liver, lung, kidney and brain. The resulting entities can be either derived from an adult stem cell population, or generated from pluripotent stem cells using a specific differentiation protocol. However, many organoid models only recapitulate the organs parenchyma but are devoid of stromal components such as blood vessels, connective tissue and inflammatory cells. Recent studies show that the incorporation of endothelial and mesenchymal cells into organoids improved their maturation and might be required to create fully functional micro-tissues, which will allow deeper insights into human embryogenesis as well as disease development and progression. In this review article, we will summarize and discuss recent works trying to incorporate stromal components into organoids, with a special focus on neural organoid models.
KW  - organoid
KW  - stroma
KW  - sasculature
KW  - neural
KW  - microglia
KW  - blood vessel
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-235628
SN  - 0300-9564
VL  - 127
ER  -