TY  - JOUR
A1  - de Vreeze, Ronald S. A.
A1  - Coevorden, Frits van
A1  - Boerrigter, Lucie
A1  - Nederlof, Petra M.
A1  - Haas, Rick L.
A1  - Bras, Johannes
A1  - Rosenwald, Andreas
A1  - Mentzel, Thomas
A1  - de Jong, Daphne
T1  - Delineation of Chondroid Lipoma: An Immunohistochemical and Molecular Biological Analysis
JF  - Sarcoma
N2  - Aims 

Chondroid lipoma (CL) is a benign tumor that mimics a variety of soft tissue tumors and is characterized by translocation (11;16). Here, we analyze CL and its histological mimics. 

Methods 

CL ( ) was compared to a variety of histological mimics ( ) for morphological aspects and immunohistochemical features including cyclinD1(CCND1). Using FISH analysis, CCND1 and FUS were investigated as potential translocation partners. 

Results 

All CLs were strongly positive for CCND1. One of 4 myoepitheliomas, CCND1, was positive. In well-differentiated lipomatous tumors and in chondrosarcomas, CCND1 was frequently expressed, but all myxoid liposarcomas were negative. FISH analysis did not give support for direct involvement of CCND1 and FUS as translocation partners. 

Conclusions 

Chondroid lipoma is extremely rare and has several and more prevalent histological mimics. The differential diagnosis of chondroid lipomas can be unraveled using immunohistochemical and molecular support.
KW  - Lipom
Y1  - 2011
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-135103
VL  - 2011
IS  - Article ID 638403
ER  - 
TY  - JOUR
A1  - Sander, Brigitta
A1  - de Jong, Daphne
A1  - Rosenwald, Andreas
A1  - Xie, Wanling
A1  - Balagué, Olga
A1  - Calaminici, Maria
A1  - Carreras, Joaquim
A1  - Gaulard, Philippe
A1  - Gribben, John
A1  - Hagenbeek, Anton
A1  - Kersten, Marie José
A1  - Molina, Thierry Jo
A1  - Lee, Abigail
A1  - Montes-Moreno, Santiago
A1  - Ott, German
A1  - Raemaekers, John
A1  - Salles, Gilles
A1  - Sehn, Laurie
A1  - Thorns, Christoph
A1  - Wahlin, Bjorn E.
A1  - Gascoyne, Randy D.
A1  - Weller, Edie
T1  - The reliability of immunohistochemical analysis of the tumor microenvironment in follicular lymphoma: a validation study from the Lunenburg Lymphoma Biomarker Consortium
JF  - Haematologica
N2  - The cellular microenvironment in follicular lymphoma is of biological and clinical importance. Studies on the clinical significance of non-malignant cell populations have generated conflicting results, which may partly be influenced by poor reproducibility in immunohistochemical marker quantification. In this study, the reproducibility of manual scoring and automated microscopy based on a tissue microarray of 25 follicular lymphomas as compared to flow cytometry is evaluated. The agreement between manual scoring and flow cytometry was moderate for CD3, low for CD4, and moderate to high for CD8, with some laboratories scoring closer to the flow cytometry results. Agreement in manual quantification across the 7 laboratories was low to moderate for CD3, CD4, CD8 and FOXP3 frequencies, moderate for CD21, low for MIB1 and CD68, and high for CD10. Manual scoring of the architectural distribution resulted in moderate agreement for CD3, CD4 and CD8, and low agreement for FOXP3 and CD68. Comparing manual scoring to automated microscopy demonstrated that manual scoring increased the variability in the low and high frequency interval with some laboratories showing a better agreement with automated scores. Manual scoring reliably identified rare architectural patterns of T-cell infiltrates. Automated microscopy analyses for T-cell markers by two different instruments were highly reproducible and provided acceptable agreement with flow cytometry. These validation results provide explanations for the heterogeneous findings on the prognostic value of the microenvironment in follicular lymphoma. We recommend a more objective measurement, such as computer-assisted scoring, in future studies of the prognostic impact of microenvironment in follicular lymphoma patients.
KW  - CD/metabolism
KW  - flow cytometry
KW  - antigens
KW  - regulatory T-cells
KW  - independent predictor
KW  - gene expression
KW  - high numbers
KW  - CD40 ligand
KW  - Riutximab
KW  - survival
KW  - marcophages
KW  - transformation
KW  - in-vitro
Y1  - 2014
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-116875
SN  - 1592-8721
VL  - 99
IS  - 4
ER  -