TY - JOUR A1 - Volpp, Linda A1 - Ferianec, Vladimír A1 - Ježovičová, Miriam A1 - Ďuračková, Zdeňka A1 - Scherf-Clavel, Oliver A1 - Högger, Petra T1 - Constituents and Metabolites of a French Oak Wood Extract (Robuvit®) in Serum and Blood Cell Samples of Women Undergoing Hysterectomy JF - Frontiers in Pharmacology N2 - Ellagitannins are signature constituents of oak wood and their consumption has been associated with various health benefits. In vivo, they undergo metabolic degradation including gut microbial metabolism yielding urolithins. Only limited data is available about compounds being present in blood after intake of an extract from French oak wood, Robuvit®. In the course of a randomized, double-blind, controlled clinical investigation, 66 patients undergoing hysterectomy received placebo or 300 mg Robuvit® per day before and over 8 weeks after surgery. Serum and blood cell samples were analyzed by liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). The number of urolithin producers and the urolithin levels increased after intake of Robuvit®. In serum samples, the median concentration of urolithin A was 14.0 ng/ml [interquartile range (IQR) 57.4] after 8 weeks. Urolithin B was determined at 22.3 ng/ml (IQR 12.6), urolithin C at 2.66 ng/ml (IQR 2.08). In blood cells, lower concentrations and only urolithins A and B were detected. A statistically significant association of lower post-surgical pain scores with metabotype A was detected (p < 0.05). To conclude, supplementation with French oak wood extract raised urolithin generation in patients and suggested health advantages for urolithin-producers. KW - polyphenols KW - urolithins KW - oak wood KW - post-surgery recovery KW - human KW - liquid chromatography electrospray ionization tandem mass spectrometry Y1 - 2020 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-200105 SN - 1663-9812 VL - 11 IS - 74 ER - TY - JOUR A1 - Ferianec, Vladimír A1 - Fülöp, Matej A1 - Ježovičová, Miriam A1 - Radošinská, Jana A1 - Husseinová, Marta A1 - Feriancová, Michaela A1 - Radošinská, Dominika A1 - Barančík, Miroslav A1 - Muchová, Jana A1 - Hȍgger, Petra A1 - Ďuračková, Zdeňka T1 - The oak−wood extract Robuvit\(^®\) improves recovery and oxidative stress after hysterectomy: a randomized, double-blind, placebo-controlled pilot study JF - Nutrients N2 - Hysterectomy has a variety of medical indications and improves pre-operative symptoms but might compromise the quality of life during recovery due to symptoms such as fatigue, headache, nausea, depression, or pain. The aim of the present study was to determine the effect of a standardized extract from French oak wood (Quercus robur) containing at least 40% polyphenols of the ellagitannins class, Robuvit\(^®\), on convalescence and oxidative stress of women after hysterectomy. Recovery status was monitored with the SF-36 questionnaire. The supplementation with Robuvit\(^®\) (300 mg/day) during 4 weeks significantly improved general and mental health, while under placebo some items significantly deteriorated. Oxidative stress and enhancement of MMP–9 activity was significantly reduced by Robuvit\(^®\) versus placebo. After 8 weeks of intervention, the patients’ condition improved independently of the intervention. Our results suggest that the use of Robuvit\(^®\) as a natural supplement relieves post-operative symptoms of patients after hysterectomy and reduces oxidative stress. The study was registered with ID ISRCTN 11457040 (13/09/2019). KW - hysterectomy KW - Robuvit\(^®\) KW - oak wood extract KW - post-operative recovery KW - oxidative stress KW - matrix metalloproteinases KW - complementary medicine Y1 - 2020 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-203265 SN - 2072-6643 VL - 12 IS - 4 ER - TY - JOUR A1 - Milić, Mirta A1 - Ceppi, Marcello A1 - Bruzzone, Marco A1 - Azqueta, Amaya A1 - Brunborg, Gunnar A1 - Godschalk, Roger A1 - Koppen, Gudrun A1 - Langie, Sabine A1 - Møller, Peter A1 - Teixeira, João Paulo A1 - Alija, Avdulla A1 - Anderson, Diana A1 - Andrade, Vanessa A1 - Andreoli, Cristina A1 - Asllani, Fisnik A1 - Bangkoglu, Ezgi Eyluel A1 - Barančoková, Magdalena A1 - Basaran, Nursen A1 - Boutet-Robinet, Elisa A1 - Buschini, Annamaria A1 - Cavallo, Delia A1 - Costa Pereira, Cristiana A1 - Costa, Carla A1 - Costa, Solange A1 - Da Silva, Juliana A1 - Del Boˊ, Cristian A1 - Dimitrijević Srećković, Vesna A1 - Djelić, Ninoslav A1 - Dobrzyńska, Malgorzata A1 - Duračková, Zdenka A1 - Dvořáková, Monika A1 - Gajski, Goran A1 - Galati, Serena A1 - García Lima, Omar A1 - Giovannelli, Lisa A1 - Goroshinskaya, Irina A. A1 - Grindel, Annemarie A1 - Gutzkow, Kristine B. A1 - Hernández, Alba A1 - Hernández, Carlos A1 - Holven, Kirsten B. A1 - Ibero-Baraibar, Idoia A1 - Ottestad, Inger A1 - Kadioglu, Ela A1 - Kažimirová, Alena A1 - Kuznetsova, Elena A1 - Ladeira, Carina A1 - Laffon, Blanca A1 - Lamonaca, Palma A1 - Lebailly, Pierre A1 - Louro, Henriqueta A1 - Mandina Cardoso, Tania A1 - Marcon, Francesca A1 - Marcos, Ricard A1 - Moretti, Massimo A1 - Moretti, Silvia A1 - Najafzadeh, Mojgan A1 - Nemeth, Zsuzsanna A1 - Neri, Monica A1 - Novotna, Bozena A1 - Orlow, Irene A1 - Paduchova, Zuzana A1 - Pastor, Susana A1 - Perdry, Hervé A1 - Spremo-Potparević, Biljana A1 - Ramadhani, Dwi A1 - Riso, Patrizia A1 - Rohr, Paula A1 - Rojas, Emilio A1 - Rossner, Pavel A1 - Safar, Anna A1 - Sardas, Semra A1 - Silva, Maria João A1 - Sirota, Nikolay A1 - Smolkova, Bozena A1 - Staruchova, Marta A1 - Stetina, Rudolf A1 - Stopper, Helga A1 - Surikova, Ekaterina I. A1 - Ulven, Stine M. A1 - Ursini, Cinzia Lucia A1 - Valdiglesias, Vanessa A1 - Valverde, Mahara A1 - Vodicka, Pavel A1 - Volkovova, Katarina A1 - Wagner, Karl-Heinz A1 - Živković, Lada A1 - Dušinská, Maria A1 - Collins, Andrew R. A1 - Bonassi, Stefano T1 - The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders JF - Mutation Research/Reviews in Mutation Research N2 - The alkaline comet assay, or single cell gel electrophoresis, is one of the most popular methods for assessing DNA damage in human population. One of the open issues concerning this assay is the identification of those factors that can explain the large inter-individual and inter-laboratory variation. International collaborative initiatives such as the hCOMET project - a COST Action launched in 2016 - represent a valuable tool to meet this challenge. The aims of hCOMET were to establish reference values for the level of DNA damage in humans, to investigate the effect of host factors, lifestyle and exposure to genotoxic agents, and to compare different sources of assay variability. A database of 19,320 subjects was generated, pooling data from 105 studies run by 44 laboratories in 26 countries between 1999 and 2019. A mixed random effect log-linear model, in parallel with a classic meta-analysis, was applied to take into account the extensive heterogeneity of data, due to descriptor, specimen and protocol variability. As a result of this analysis interquartile intervals of DNA strand breaks (which includes alkali-labile sites) were reported for tail intensity, tail length, and tail moment (comet assay descriptors). A small variation by age was reported in some datasets, suggesting higher DNA damage in oldest age-classes, while no effect could be shown for sex or smoking habit, although the lack of data on heavy smokers has still to be considered. Finally, highly significant differences in DNA damage were found for most exposures investigated in specific studies. In conclusion, these data, which confirm that DNA damage measured by the comet assay is an excellent biomarker of exposure in several conditions, may contribute to improving the quality of study design and to the standardization of results of the comet assay in human populations. KW - comet assay KW - DNA damage KW - pooled analysis KW - human biomonitoring KW - biomarkers Y1 - 2021 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-371614 VL - 787 ER -