TY  - JOUR
A1  - Kunz, Tobias C.
A1  - Rühling, Marcel
A1  - Moldovan, Adriana
A1  - Paprotka, Kerstin
A1  - Kozjak-Pavlovic, Vera
A1  - Rudel, Thomas
A1  - Fraunholz, Martin
T1  - The Expandables: Cracking the Staphylococcal Cell Wall for Expansion Microscopy
JF  - Frontiers in Cellular and Infection Microbiology
N2  - Expansion Microscopy (ExM) is a novel tool improving the resolution of fluorescence microscopy by linking the sample into a hydrogel that gets physically expanded in water. Previously, we have used ExM to visualize the intracellular Gram-negative pathogens Chlamydia trachomatis, Simkania negevensis, and Neisseria gonorrhoeae. Gram-positive bacteria have a rigid and thick cell wall that impedes classic expansion strategies. Here we developed an approach, which included a series of enzymatic treatments resulting in isotropic 4× expansion of the Gram-positive pathogen Staphylococcus aureus. We further demonstrate the suitability of the technique for imaging of planktonic bacteria as well as endocytosed, intracellular bacteria at a spatial resolution of approximately 60 nm with conventional confocal laser scanning microscopy.
KW  - high-resolution imaging
KW  - endosomes
KW  - autophagosomes
KW  - host-pathogen interaction
KW  - expansion microscopy
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-232292
SN  - 2235-2988
VL  - 11
ER  -