TY  - JOUR
A1  - Eltamany, Enas E.
A1  - Abdelmohsen, Usama Ramadan
A1  - Hal, Dina M.
A1  - Ibrahim, Amany K.
A1  - Hassanean, Hashim A.
A1  - Abdelhameed, Reda F. A.
A1  - Temraz, Tarek A.
A1  - Hajjar, Dina
A1  - Makki, Arwa A.
A1  - Hendawy, Omnia Magdy
A1  - AboulMagd, Asmaa M.
A1  - Youssif, Khayrya A.
A1  - Bringmann, Gerhard
A1  - Ahmed, Safwat A.
T1  - Holospiniferoside: A New Antitumor Cerebroside from The Red Sea Cucumber Holothuria spinifera: In Vitro and In Silico Studies
JF  - Molecules
N2  - Chemical investigation of the methanolic extract of the Red Sea cucumber Holothuria spinifera led to the isolation of a new cerebroside, holospiniferoside (1), together with thymidine (2), methyl-α-d-glucopyranoside (3), a new triacylglycerol (4), and cholesterol (5). Their chemical structures were established by NMR and mass spectrometric analysis, including gas chromatography–mass spectrometry (GC–MS) and high-resolution mass spectrometry (HRMS). All the isolated compounds are reported in this species for the first time. Moreover, compound 1 exhibited promising in vitro antiproliferative effect on the human breast cancer cell line (MCF-7) with IC\(_{50}\) of 20.6 µM compared to the IC50 of 15.3 µM for the drug cisplatin. To predict the possible mechanism underlying the cytotoxicity of compound 1, a docking study was performed to elucidate its binding interactions with the active site of the protein Mdm2–p53. Compound 1 displayed an apoptotic activity via strong interaction with the active site of the target protein. This study highlights the importance of marine natural products in the design of new anticancer agents.
KW  - Holothuria spinifera
KW  - HRMS
KW  - cerebrosides
KW  - molecular docking
KW  - cytotoxicity
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-234058
SN  - 1420-3049
VL  - 26
IS  - 6
ER  -