TY  - JOUR
A1  - Machata, Silke
A1  - Sreekantapuram, Sravya
A1  - Hünniger, Kerstin
A1  - Kurzai, Oliver
A1  - Dunker, Christine
A1  - Schubert, Katja
A1  - Krüger, Wibke
A1  - Schulze-Richter, Bianca
A1  - Speth, Cornelia
A1  - Rambach, Günter
A1  - Jacobsen, Ilse D.
T1  - Significant Differences in Host-Pathogen Interactions Between Murine and Human Whole Blood
JF  - Frontiers in Immunology
N2  - Murine infection models are widely used to study systemic candidiasis caused by C. albicans. Whole-blood models can help to elucidate host-pathogens interactions and have been used for several Candida species in human blood. We adapted the human whole-blood model to murine blood. Unlike human blood, murine blood was unable to reduce fungal burden and more substantial filamentation of C. albicans was observed. This coincided with less fungal association with leukocytes, especially neutrophils. The lower neutrophil number in murine blood only partially explains insufficient infection and filamentation control, as spiking with murine neutrophils had only limited effects on fungal killing. Furthermore, increased fungal survival is not mediated by enhanced filamentation, as a filament-deficient mutant was likewise not eliminated. We also observed host-dependent differences for interaction of platelets with C. albicans, showing enhanced platelet aggregation, adhesion and activation in murine blood. For human blood, opsonization was shown to decrease platelet interaction suggesting that complement factors interfere with fungus-to-platelet binding. Our results reveal substantial differences between murine and human whole-blood models infected with C. albicans and thereby demonstrate limitations in the translatability of this ex vivo model between hosts.
KW  - whole blood ex vivo model
KW  - host-pathogen interaction
KW  - neutrophils
KW  - mice
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-222575
SN  - 1664-3224
VL  - 11
ER  -