TY  - JOUR
A1  - Dreschers, Stephan
A1  - Saupp, Peter
A1  - Hornef, Mathias
A1  - Prehn, Andrea
A1  - Platen, Christopher
A1  - Morschhäuser, Joachim
A1  - Orlikowsky, Thorsten W.
T1  - Reduced PICD in Monocytes Mounts Altered Neonate Immune Response to Candida albicans
JF  - PLoS ONE
N2  - Background
Invasive fungal infections with Candida albicans (C. albicans) occur frequently in extremely low birthweight (ELBW) infants and are associated with poor outcome. Phagocytosis of C.albicans initializes apoptosis in monocytes (phagocytosis induced cell death, PICD). PICD is reduced in neonatal cord blood monocytes (CBMO).

Hypothesis
Phagocytosis of C. albicans causes PICD which differs between neonatal monocytes (CBMO) and adult peripheral blood monocytes (PBMO) due to lower stimulation of TLR-mediated immune responses.

Methods
The ability to phagocytose C. albicans, expression of TLRs, the induction of apoptosis (assessment of sub-G1 and nick-strand breaks) were analyzed by FACS. TLR signalling was induced by agonists such as lipopolysaccharide (LPS), Pam3Cys, FSL-1 and Zymosan and blocked (neutralizing TLR2 antibodies and MYD88 inhibitor).

Results
Phagocytic indices of PBMO and CBMO were similar. Following stimulation with agonists and C. albicans induced up-regulation of TLR2 and consecutive phosphorylation of MAP kinase P38 and expression of TNF-α, which were stronger on PBMO compared to CBMO (p < 0.005). Downstream, TLR2 signalling initiated caspase-3-dependent PICD which was found reduced in CBMO (p < 0.05 vs PBMO).

Conclusion
Our data suggest direct involvement of TLR2-signalling in C. albicans-induced PICD in monocytes and an alteration of this pathway in CBMO.
KW  - Candida albicans
KW  - monocytes
KW  - immune response
KW  - PICD
Y1  - 2016
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-166778
VL  - 11
IS  - 11
ER  - 
TY  - JOUR
A1  - Holzmann-Littig, Christopher
A1  - Stadler, David
A1  - Popp, Maria
A1  - Kranke, Peter
A1  - Fichtner, Falk
A1  - Schmaderer, Christoph
A1  - Renders, Lutz
A1  - Braunisch, Matthias Christoph
A1  - Assali, Tarek
A1  - Platen, Louise
A1  - Wijnen-Meijer, Marjo
A1  - Lühnen, Julia
A1  - Steckelberg, Anke
A1  - Pfadenhauer, Lisa
A1  - Haller, Bernhard
A1  - Fuetterer, Cornelia
A1  - Seeber, Christian
A1  - Schaaf, Christian
T1  - Locating medical information during an infodemic: information seeking behavior and strategies of health-care workers in Germany
JF  - Healthcare
N2  - Background: The COVID-19 pandemic has led to a flood of — often contradictory — evidence. HCWs had to develop strategies to locate information that supported their work. We investigated the information-seeking of different HCW groups in Germany. Methods: In December 2020, we conducted online surveys on COVID-19 information sources, strategies, assigned trustworthiness, and barriers — and in February 2021, on COVID-19 vaccination information sources. Results were analyzed descriptively; group comparisons were performed using χ\(^2\)-tests. Results: For general COVID-19-related medical information (413 participants), non-physicians most often selected official websites (57%), TV (57%), and e-mail/newsletters (46%) as preferred information sources — physicians chose official websites (63%), e-mail/newsletters (56%), and professional journals (55%). Non-physician HCWs used Facebook/YouTube more frequently. The main barriers were insufficient time and access issues. Non-physicians chose abstracts (66%), videos (45%), and webinars (40%) as preferred information strategy; physicians: overviews with algorithms (66%), abstracts (62%), webinars (48%). Information seeking on COVID-19 vaccination (2700 participants) was quite similar, however, with newspapers being more often used by non-physicians (63%) vs. physician HCWs (70%). Conclusion: Non-physician HCWs more often consulted public information sources. Employers/institutions should ensure the supply of professional, targeted COVID-19 information for different HCW groups.
KW  - COVID-19
KW  - infodemic
KW  - health-care workers
KW  - HCW
KW  - information strategies
KW  - emergency information
Y1  - 2023
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-319306
SN  - 2227-9032
VL  - 11
IS  - 11
ER  -