TY  - JOUR
A1  - Berger, Susanne
A1  - Ellersiek, Ulrike
A1  - Kinzelt, Dagmar
A1  - Steinmüller, Klaus
T1  - Immunopurification of a subcomplex of the NAD(P)H-plastoquinone-oxidoreductase from the cyanobacterium Synechocystis sp. PCC6803
N2  - An antibody against the NDH-K subunit of the NAD(P)H-dehydrogenase from the cyanobacterium Synechocystis sp. PCC6803 was used to isolate a subcomplex ofthe enzyme from Triton X-lOO solubilized total membranes by immunoaffinity chromatography. The isolated subcomplex consisted of seven major polypeptides with molecular masses of 43, 27, 24, 21, 18, 14 and 7 kDa. The amino-terminal amino acid sequences of the polypeptides were determined. By comparing the sequences with the amino acid sequences deduced from DNA. three proteins were identified as NDH-H (43 kDa). NDH-K (27 kDa) and NDH-J (24 kDa). A fourth subunit (NDH-J, 21 kDa) was identified by Western blot analysis with an NDH-J antibody.
KW  - NAD(P)H-dehydrogenase
KW  - Cyanobacteria
KW  - Synechocystis sp. PCC6803
KW  - NDH-H
KW  - NDH-I
KW  - NDH-J
KW  - NDH-K
Y1  - 1993
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-31249
ER  -