TY  - JOUR
A1  - Eltamany, Enas E.
A1  - Abdelmohsen, Usama Ramadan
A1  - Hal, Dina M.
A1  - Ibrahim, Amany K.
A1  - Hassanean, Hashim A.
A1  - Abdelhameed, Reda F. A.
A1  - Temraz, Tarek A.
A1  - Hajjar, Dina
A1  - Makki, Arwa A.
A1  - Hendawy, Omnia Magdy
A1  - AboulMagd, Asmaa M.
A1  - Youssif, Khayrya A.
A1  - Bringmann, Gerhard
A1  - Ahmed, Safwat A.
T1  - Holospiniferoside: A New Antitumor Cerebroside from The Red Sea Cucumber Holothuria spinifera: In Vitro and In Silico Studies
JF  - Molecules
N2  - Chemical investigation of the methanolic extract of the Red Sea cucumber Holothuria spinifera led to the isolation of a new cerebroside, holospiniferoside (1), together with thymidine (2), methyl-α-d-glucopyranoside (3), a new triacylglycerol (4), and cholesterol (5). Their chemical structures were established by NMR and mass spectrometric analysis, including gas chromatography–mass spectrometry (GC–MS) and high-resolution mass spectrometry (HRMS). All the isolated compounds are reported in this species for the first time. Moreover, compound 1 exhibited promising in vitro antiproliferative effect on the human breast cancer cell line (MCF-7) with IC\(_{50}\) of 20.6 µM compared to the IC50 of 15.3 µM for the drug cisplatin. To predict the possible mechanism underlying the cytotoxicity of compound 1, a docking study was performed to elucidate its binding interactions with the active site of the protein Mdm2–p53. Compound 1 displayed an apoptotic activity via strong interaction with the active site of the target protein. This study highlights the importance of marine natural products in the design of new anticancer agents.
KW  - Holothuria spinifera
KW  - HRMS
KW  - cerebrosides
KW  - molecular docking
KW  - cytotoxicity
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-234058
SN  - 1420-3049
VL  - 26
IS  - 6
ER  - 
TY  - JOUR
A1  - Abdelhameed, Reda F. A.
A1  - Eltamany, Enas E.
A1  - Hal, Dina M.
A1  - Ibrahim, Amany K.
A1  - AboulMagd, Asmaa M.
A1  - Al-Warhi, Tarfah
A1  - Youssif, Khayrya A.
A1  - Abd El-kader, Adel M.
A1  - Hassanean, Hashim A.
A1  - Fayez, Shaimaa
A1  - Bringmann, Gerhard
A1  - Ahmed, Safwat A.
A1  - Abdelmohsen, Usama Ramadan
T1  - New cytotoxic cerebrosides from the Red Sea cucumber Holothuria spinifera supported by in-silico studies
JF  - Marine Drugs
N2  - Bioactivity-guided fractionation of a methanolic extract of the Red Sea cucumber Holothuria spinifera and LC-HRESIMS-assisted dereplication resulted in the isolation of four compounds, three new cerebrosides, spiniferosides A (1), B (2), and C (3), and cholesterol sulfate (4). The chemical structures of the isolated compounds were established on the basis of their 1D NMR and HRMS spectral data. Metabolic profiling of the H. spinifera extract indicated the presence of diverse secondary metabolites, mostly hydroxy fatty acids, diterpenes, triterpenes, and cerebrosides. The isolated compounds were tested for their in vitro cytotoxicities against the breast adenocarcinoma MCF-7 cell line. Compounds 1, 2, 3, and 4 displayed promising cytotoxic activities against MCF-7 cells, with IC\(_{50}\) values of 13.83, 8.13, 8.27, and 35.56 µM, respectively, compared to that of the standard drug doxorubicin (IC\(_{50}\) 8.64 µM). Additionally, docking studies were performed for compounds 1, 2, 3, and 4 to elucidate their binding interactions with the active site of the SET protein, an inhibitor of protein phosphatase 2A (PP2A), which could explain their cytotoxic activity. This study highlights the important role of these metabolites in the defense mechanism of the sea cucumber against fouling organisms and the potential uses of these active molecules in the design of new anticancer agents.
KW  - LC-HRESIMS
KW  - Holothuria spinifera
KW  - cerebrosides
KW  - molecular docking
KW  - cytotoxicity
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-211089
SN  - 1660-3397
VL  - 18
IS  - 8
ER  -