TY  - JOUR
A1  - Herrmann, Marietta
A1  - Engelke, Klaus
A1  - Ebert, Regina
A1  - Müller-Deubert, Sigrid
A1  - Rudert, Maximilian
A1  - Ziouti, Fani
A1  - Jundt, Franziska
A1  - Felsenberg, Dieter
A1  - Jakob, Franz
T1  - Interactions between muscle and bone — Where physics meets biology
JF  - Biomolecules
N2  - Muscle and bone interact via physical forces and secreted osteokines and myokines. Physical forces are generated through gravity, locomotion, exercise, and external devices. Cells sense mechanical strain via adhesion molecules and translate it into biochemical responses, modulating the basic mechanisms of cellular biology such as lineage commitment, tissue formation, and maturation. This may result in the initiation of bone formation, muscle hypertrophy, and the enhanced production of extracellular matrix constituents, adhesion molecules, and cytoskeletal elements. Bone and muscle mass, resistance to strain, and the stiffness of matrix, cells, and tissues are enhanced, influencing fracture resistance and muscle power. This propagates a dynamic and continuous reciprocity of physicochemical interaction. Secreted growth and differentiation factors are important effectors of mutual interaction. The acute effects of exercise induce the secretion of exosomes with cargo molecules that are capable of mediating the endocrine effects between muscle, bone, and the organism. Long-term changes induce adaptations of the respective tissue secretome that maintain adequate homeostatic conditions. Lessons from unloading, microgravity, and disuse teach us that gratuitous tissue is removed or reorganized while immobility and inflammation trigger muscle and bone marrow fatty infiltration and propagate degenerative diseases such as sarcopenia and osteoporosis. Ongoing research will certainly find new therapeutic targets for prevention and treatment.
KW  - muscle
KW  - bone
KW  - mechanosensing
KW  - mechanotransduction
KW  - myokines
KW  - osteokines adaptation
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-203399
SN  - 2218-273X
VL  - 10
IS  - 3
ER  - 
TY  - JOUR
A1  - Winkelbeiner, Nicola
A1  - Wandt, Viktoria K.
A1  - Ebert, Franziska
A1  - Lossow, Kristina
A1  - Bankoglu, Ezgi E.
A1  - Martin, Maximilian
A1  - Mangerich, Aswin
A1  - Stopper, Helga
A1  - Bornhorst, Julia
A1  - Kipp, Anna P.
A1  - Schwerdtle, Tanja
T1  - A multi-endpoint approach to base excision repair incision activity augmented by PARylation and DNA damage levels in mice: impact of sex and age
JF  - International Journal of Molecular Sciences
N2  - Investigation of processes that contribute to the maintenance of genomic stability is one crucial factor in the attempt to understand mechanisms that facilitate ageing. The DNA damage response (DDR) and DNA repair mechanisms are crucial to safeguard the integrity of DNA and to prevent accumulation of persistent DNA damage. Among them, base excision repair (BER) plays a decisive role. BER is the major repair pathway for small oxidative base modifications and apurinic/apyrimidinic (AP) sites. We established a highly sensitive non-radioactive assay to measure BER incision activity in murine liver samples. Incision activity can be assessed towards the three DNA lesions 8-oxo-2'-deoxyguanosine (8-oxodG), 5-hydroxy-2'-deoxyuracil (5-OHdU), and an AP site analogue. We applied the established assay to murine livers of adult and old mice of both sexes. Furthermore, poly(ADP-ribosyl)ation (PARylation) was assessed, which is an important determinant in DDR and BER. Additionally, DNA damage levels were measured to examine the overall damage levels. No impact of ageing on the investigated endpoints in liver tissue were found. However, animal sex seems to be a significant impact factor, as evident by sex-dependent alterations in all endpoints investigated. Moreover, our results revealed interrelationships between the investigated endpoints indicative for the synergetic mode of action of the cellular DNA integrity maintaining machinery.
KW  - maintenance of genomic integrity
KW  - ageing
KW  - sex
KW  - DNA damage
KW  - base excision repair (incision activity)
KW  - DNA damage response
KW  - poly(ADP-ribosyl)ation
KW  - liver
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-285706
SN  - 1422-0067
VL  - 21
IS  - 18
ER  - 
TY  - JOUR
A1  - Maichl, Daniela Simone
A1  - Kirner, Julius Arthur
A1  - Beck, Susanne
A1  - Cheng, Wen-Hui
A1  - Krug, Melanie
A1  - Kuric, Martin
A1  - Ade, Carsten Patrick
A1  - Bischler, Thorsten
A1  - Jakob, Franz
A1  - Hose, Dirk
A1  - Seckinger, Anja
A1  - Ebert, Regina
A1  - Jundt, Franziska
T1  - Identification of NOTCH-driven matrisome-associated genes as prognostic indicators of multiple myeloma patient survival
JF  - Blood Cancer Journal
N2  - No abstract available.
KW  - cancer microenvironment
KW  - myeloma
Y1  - 2023
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-357598
VL  - 13
ER  -