TY  - JOUR
A1  - Korkmaz, Yüksel
A1  - Puladi, Behrus
A1  - Galler, Kerstin
A1  - Kämmerer, Peer W.
A1  - Schröder, Agnes
A1  - Gölz, Lina
A1  - Sparwasser, Tim
A1  - Bloch, Wilhelm
A1  - Friebe, Andreas
A1  - Deschner, James
T1  - Inflammation in the human periodontium induces downregulation of the α\(_1\)- and β\(_1\)-subunits of the sGC in cementoclasts
JF  - International Journal of Molecular Sciences
N2  - Nitric oxide (NO) binds to soluble guanylyl cyclase (sGC), activates it in a reduced oxidized heme iron state, and generates cyclic Guanosine Monophosphate (cGMP), which results in vasodilatation and inhibition of osteoclast activity. In inflammation, sGC is oxidized and becomes insensitive to NO. NO- and heme-independent activation of sGC requires protein expression of the α\(_1\)- and β\(_1\)-subunits. Inflammation of the periodontium induces the resorption of cementum by cementoclasts and the resorption of the alveolar bone by osteoclasts, which can lead to tooth loss. As the presence of sGC in cementoclasts is unknown, we investigated the α\(_1\)- and β\(_1\)-subunits of sGC in cementoclasts of healthy and inflamed human periodontium using double immunostaining for CD68 and cathepsin K and compared the findings with those of osteoclasts from the same sections. In comparison to cementoclasts in the healthy periodontium, cementoclasts under inflammatory conditions showed a decreased staining intensity for both α\(_1\)- and β\(_1\)-subunits of sGC, indicating reduced protein expression of these subunits. Therefore, pharmacological activation of sGC in inflamed periodontal tissues in an NO- and heme-independent manner could be considered as a new treatment strategy to inhibit cementum resorption.
KW  - nitric oxide
KW  - soluble guanylyl cyclase
KW  - cGMP
KW  - cementoclasts
KW  - cementum
KW  - osteoclasts
KW  - alveolar bone
KW  - periodontitis
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-285783
SN  - 1422-0067
VL  - 22
IS  - 2
ER  -