TY - THES A1 - Geiger, Markus T1 - The Geology of the southern Warmbad Basin Margin - Tephrostratigraphy, Age, Fossil Record and Sedimentary Environment of Carboniferous-Permian Glacigenic Deposits of the Dwyka Group, Zwartbas, southern Namibia N2 - At Zwartbas, about 10 km west of Vioolsdrif, southern Namibia, the Dwyka succession is composed of tillites and distal fossiliferous dropstone-bearing glacio-marine shales. The completely exposed Dwyka succession is interbedded with thin bentonites, altered distal pyroclastic deposits, which were derived from the magmatic arc at the southern rim of Gondwana. Dropstone-bearing and dropstonefree sequences intercalate with four diamictites, of which the two lowest were certainly recognised as tillites. Four events of deglaciation were proven at Zwartbas and thus consist with correlative deposits in southern Africa. Numerous fossilised fishes, trace fossils, and plant fragments appear frequently within the lower half of the Dwyka succession whereas trace fossils were principally found in the complete succession. Although the environmental determination is quite problematic, the fossil assemblage rather implies proximal, shallow water conditions with temporary restricted oxygenation. The hinterland was covered with considerable vegetation, which points to a moderate climate. Water salinity determinations based on shale geochemistry rectify contrary palaeontological results and point to rather brackish or non-marine conditions in comparison to present-day salinites. Geochemical analyses of the bentonites relate the pyroclastic deposits with acid to intermediate source magmas, as they are known from the magmatic arc in present-day Patagonia. Tectono-magmatic comparisons furthermore emphasise a syn-collision or volcanic-arc situation of the magma source. However, significant cyclicity in the production of the pyroclastic deposits was not observed. Radiometric age determinations of two tuff beds clearly date the onset of glacial activity into the Late Carboniferous. KW - Namibia KW - Karbon KW - Permokarbon KW - Vereisung KW - Tuff KW - Oranje KW - Fossil KW - Geochemie KW - Schwermineral KW - Diamiktit KW - Tillit KW - Tonstein KW - Dwyka KW - Carboniferous KW - Permian KW - Glaciation KW - Geochemistry KW - Petrography Y1 - 2000 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-46251 ER - TY - THES A1 - Geiger, Markus T1 - An Explanation of the Geological Map 1:10000 of the Namibian borderland along the Orange River at Zwartbas - Warmbad District - Karas Region - Namibia N2 - The locality of Zwartbas is situated at the border of Namibia and South Africa about 15 km west of Noordoewer. The mapped area is confined by the Tandjieskoppe Mountains in the north and the Orange River in the south. Outcropping rocks are predominantly sediments of the Nama Group and of the Karoo Supergroup. During the compilation of this paper doubts arose about the correct classification of the Nama rocks as it is found in literature. Since no certain clues were found to revise the classification of the Nama rocks, the original classification remains still valid. Thus the Kuibis and Schwarzrand Subgroup constitute the Nama succession and date it to Vendian age. A glacial unconformity represents a hiatus for about 260 Ma. This is covered by sediments of the Karoo Supergroup. Late Carboniferous and early Permian glacial deposits of diamictitic shale of the Dwyka and shales of the Ecca Group overlie the unconformity. The shales of the Dwyka Group contain fossiliferous units and volcanic ash-layers. A sill of the Jurassic Tandjiesberg Dolerite Complex (also Karoo Supergroup) intruded rocks at the Dwyka-Ecca-boundary. Finally fluvial and aeolian deposits and calcretes of the Cretaceous to Tertiary Kalahari Group and recent depositionary events cover the older rocks occasionally. N2 - Die Lokalität Zwartbas liegt an der namibisch-südafrikanischen Grenze, etwa 15 km westlich von Noordoewer. Das Kartiergebiet wird durch die Tandjiesberge im Norden und den Oranje Fluß im Süden begrenzt. Die anstehenden Gesteine bestehen hauptsächlich aus Sedimenten der Nama Gruppe und der Karoo Supergruppe. Während der Erarbeitung dieser Abhandlung entstanden Zweifel an der Klassifikation der Nama Gesteine, so wie sie in der Literatur zu finden ist. Da keine sicheren Hinweise zur Revision der Klassifikation der Nama Gesteine gefunden wurden, bleibt die ursprünglich Klassifikation jedoch gültig. Die Kuibis und Schwarzrand Untergruppe bilden also die Nama Abfolge und datieren sie ins Vendian. Eine glaziale Diskontinuität repräsentiert einen Hiatus von etwa 260 Mio Jahren. Sie wird überlagert von Sedimenten der Karoo Supergruppe. Spät-karbone und früh-permische glaziale Ablagerungen von diamiktitischen Tonsteinen der Dwyka Gruppe und Tonsteine der Ecca Gruppe liegen über dieser Diskontinuität. Die Sedimente der Dwyka Gruppe sind fossilführend und enthalten Tufflagen. Ein Sill des jurassischen Tandjiesberg Dolerit Komplex (auch Karoo Supergruppe) intrudierte in die Gesteine an der Dwyka-Ecca Grenze. Schließlich bedecken lokal fluviatile und äolische Ablagerungen und Kalkkrusten der kretazischen und tertiären Kalahari Gruppe und jüngerer Ablagerungsereignisse die älteren Gesteine. KW - Namibia KW - Karbon KW - Permokarbon KW - Vereisung KW - Tuff KW - Oranje KW - Fossil KW - Geochemie KW - Schwermineral KW - Diamiktit KW - Tillit KW - Tonstein KW - Präkambrium KW - Geologische Kartierung KW - Nama KW - Karoo KW - Dwyka KW - Mapping KW - glaciation Y1 - 1999 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-46269 ER - TY - JOUR A1 - Brenner, Daniela A1 - Geiger, Nina A1 - Schlegel, Jan A1 - Diesendorf, Viktoria A1 - Kersting, Louise A1 - Fink, Julian A1 - Stelz, Linda A1 - Schneider-Schaulies, Sibylle A1 - Sauer, Markus A1 - Bodem, Jochen A1 - Seibel, Jürgen T1 - Azido-ceramides, a tool to analyse SARS-CoV-2 replication and inhibition — SARS-CoV-2 is inhibited by ceramides JF - International Journal of Molecular Sciences N2 - Recently, we have shown that C6-ceramides efficiently suppress viral replication by trapping the virus in lysosomes. Here, we use antiviral assays to evaluate a synthetic ceramide derivative α-NH2-ω-N3-C6-ceramide (AKS461) and to confirm the biological activity of C6-ceramides inhibiting SARS-CoV-2. Click-labeling with a fluorophore demonstrated that AKS461 accumulates in lysosomes. Previously, it has been shown that suppression of SARS-CoV-2 replication can be cell-type specific. Thus, AKS461 inhibited SARS-CoV-2 replication in Huh-7, Vero, and Calu-3 cells up to 2.5 orders of magnitude. The results were confirmed by CoronaFISH, indicating that AKS461 acts comparable to the unmodified C6-ceramide. Thus, AKS461 serves as a tool to study ceramide-associated cellular and viral pathways, such as SARS-CoV-2 infections, and it helped to identify lysosomes as the central organelle of C6-ceramides to inhibit viral replication. KW - ceramides KW - SARS-CoV-2 KW - azido-ceramides KW - sphingolipids Y1 - 2023 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-313581 SN - 1422-0067 VL - 24 IS - 8 ER - TY - JOUR A1 - Geiger, Nina A1 - Kersting, Louise A1 - Schlegel, Jan A1 - Stelz, Linda A1 - Fähr, Sofie A1 - Diesendorf, Viktoria A1 - Roll, Valeria A1 - Sostmann, Marie A1 - König, Eva-Maria A1 - Reinhard, Sebastian A1 - Brenner, Daniela A1 - Schneider-Schaulies, Sibylle A1 - Sauer, Markus A1 - Seibel, Jürgen A1 - Bodem, Jochen T1 - The acid ceramidase is a SARS-CoV-2 host factor JF - Cells N2 - SARS-CoV-2 variants such as the delta or omicron variants, with higher transmission rates, accelerated the global COVID-19 pandemic. Thus, novel therapeutic strategies need to be deployed. The inhibition of acid sphingomyelinase (ASM), interfering with viral entry by fluoxetine was reported. Here, we described the acid ceramidase as an additional target of fluoxetine. To discover these effects, we synthesized an ASM-independent fluoxetine derivative, AKS466. High-resolution SARS-CoV-2–RNA FISH and RTqPCR analyses demonstrate that AKS466 down-regulates viral gene expression. It is shown that SARS-CoV-2 deacidifies the lysosomal pH using the ORF3 protein. However, treatment with AKS488 or fluoxetine lowers the lysosomal pH. Our biochemical results show that AKS466 localizes to the endo-lysosomal replication compartments of infected cells, and demonstrate the enrichment of the viral genomic, minus-stranded RNA and mRNAs there. Both fluoxetine and AKS466 inhibit the acid ceramidase activity, cause endo-lysosomal ceramide elevation, and interfere with viral replication. Furthermore, Ceranib-2, a specific acid ceramidase inhibitor, reduces SARS-CoV-2 replication and, most importantly, the exogenous supplementation of C6-ceramide interferes with viral replication. These results support the hypotheses that the acid ceramidase is a SARS-CoV-2 host factor. KW - SARS-CoV-2 KW - ceramides KW - ceramidase KW - fluoxetine KW - acid sphingomyelinase Y1 - 2022 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-286105 SN - 2073-4409 VL - 11 IS - 16 ER -