TY  - JOUR
A1  - Ryma, Matthias
A1  - Tylek, Tina
A1  - Liebscher, Julia
A1  - Blum, Carina
A1  - Fernandez, Robin
A1  - Böhm, Christoph
A1  - Kastenmüller, Wolfgang
A1  - Gasteiger, Georg
A1  - Groll, Jürgen
T1  - Translation of collagen ultrastructure to biomaterial fabrication for material-independent but highly efficient topographic immunomodulation
JF  - Advanced materials
N2  - Supplement-free induction of cellular differentiation and polarization solely through the topography of materials is an auspicious strategy but has so far significantly lagged behind the efficiency and intensity of media-supplementation-based protocols. Consistent with the idea that 3D structural motifs in the extracellular matrix possess immunomodulatory capacity as part of the natural healing process, it is found in this study that human-monocyte-derived macrophages show a strong M2a-like prohealing polarization when cultured on type I rat-tail collagen fibers but not on collagen I films. Therefore, it is hypothesized that highly aligned nanofibrils also of synthetic polymers, if packed into larger bundles in 3D topographical biomimetic similarity to native collagen I, would induce a localized macrophage polarization. For the automated fabrication of such bundles in a 3D printing manner, the strategy of “melt electrofibrillation” is pioneered by the integration of flow-directed polymer phase separation into melt electrowriting and subsequent selective dissolution of the matrix polymer postprocessing. This process yields nanofiber bundles with a remarkable structural similarity to native collagen I fibers, particularly for medical-grade poly(ε-caprolactone). These biomimetic fibrillar structures indeed induce a pronounced elongation of human-monocyte-derived macrophages and unprecedentedly trigger their M2-like polarization similar in efficacy as interleukin-4 treatment.
KW  - biofabrication
KW  - extracellular matrix
KW  - immunomodulation
KW  - macrophages
KW  - melt electrofibrillation
KW  - melt electrowriting
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-256381
VL  - 33
IS  - 33
ER  -