TY  - JOUR
A1  - Schmitt, Andrea
A1  - Tatsch, Laura
A1  - Vollhardt, Alisa
A1  - Schneider-Axmann, Thomas
A1  - Raabe, Florian J.
A1  - Roell, Lukas
A1  - Heinsen, Helmut
A1  - Hof, Patrick R.
A1  - Falkai, Peter
A1  - Schmitz, Christoph
T1  - Decreased oligodendrocyte number in hippocampal subfield CA4 in schizophrenia: a replication study
JF  - Cells
N2  - Hippocampus-related cognitive deficits in working and verbal memory are frequent in schizophrenia, and hippocampal volume loss, particularly in the cornu ammonis (CA) subregions, was shown by magnetic resonance imaging studies. However, the underlying cellular alterations remain elusive. By using unbiased design-based stereology, we reported a reduction in oligodendrocyte number in CA4 in schizophrenia and of granular neurons in the dentate gyrus (DG). Here, we aimed to replicate these findings in an independent sample. We used a stereological approach to investigate the numbers and densities of neurons, oligodendrocytes, and astrocytes in CA4 and of granular neurons in the DG of left and right hemispheres in 11 brains from men with schizophrenia and 11 brains from age- and sex-matched healthy controls. In schizophrenia, a decreased number and density of oligodendrocytes was detected in the left and right CA4, whereas mean volumes of CA4 and the DG and the numbers and density of neurons, astrocytes, and granular neurons were not different in patients and controls, even after adjustment of variables because of positive correlations with postmortem interval and age. Our results replicate the previously described decrease in oligodendrocytes bilaterally in CA4 in schizophrenia and point to a deficit in oligodendrocyte maturation or a loss of mature oligodendrocytes. These changes result in impaired myelination and neuronal decoupling, both of which are linked to altered functional connectivity and subsequent cognitive dysfunction in schizophrenia.
KW  - schizophrenia
KW  - hippocampus
KW  - CA4
KW  - dentate gyrus
KW  - postmortem
KW  - stereology
KW  - oligodendrocyte
KW  - neuron
Y1  - 2022
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-290360
SN  - 2073-4409
VL  - 11
IS  - 20
ER  - 
TY  - JOUR
A1  - Bohnert, Simone
A1  - Trella, Stefanie
A1  - Preiß, Ulrich
A1  - Heinsen, Helmut
A1  - Bohnert, Michael
A1  - Zwirner, Johann
A1  - Tremblay, Marie-Ève
A1  - Monoranu, Camelia-Maria
A1  - Ondruschka, Benjamin
T1  - Density of TMEM119-positive microglial cells in postmortem cerebrospinal fluid as a surrogate marker for assessing complex neuropathological processes in the CNS
JF  - International Journal of Legal Medicine
N2  - Routine coronal paraffin-sections through the dorsal frontal and parieto-occipital cortex of a total of sixty cases with divergent causes of death were immunohistochemically (IHC) stained with an antibody against TMEM119. Samples of cerebrospinal fluid (CSF) of the same cases were collected by suboccipital needle-puncture, subjected to centrifugation and processed as cytospin preparations stained with TMEM119. Both, cytospin preparations and sections were subjected to computer-assisted density measurements. The density of microglial TMEM119-positive cortical profiles correlated with that of cytospin results and with the density of TMEM119-positive microglial profiles in the medullary layer. There was no statistically significant correlation between the density of medullary TMEM119-positive profiles and the cytospin data. Cortical microglial cells were primarily encountered in supragranular layers I, II, and IIIa and in infragranular layers V and VI, the region of U-fibers and in circumscribed foci or spread in a diffuse manner and high density over the white matter. We have evidence that cortical microglia directly migrate into CSF without using the glympathic pathway. Microglia in the medullary layer shows a strong affinity to the adventitia of deep vessels in the myelin layer. Selected rapidly fatal cases including myocardial infarcts and drowning let us conclude that microglia in cortex and myelin layer can react rapidly and its reaction and migration is subject to pre-existing external and internal factors. Cytospin preparations proved to be a simple tool to analyze and assess complex changes in the CNS after rapid fatal damage. There is no statistically significant correlation between cytospin and postmortem interval. Therefore, the quantitative analyses of postmortem cytospins obviously reflect the neuropathology of the complete central nervous system. Cytospins provide forensic pathologists a rather simple and easy to perform method for the global assessment of CNS affliction.
KW  - cerebrospinal fluid
KW  - forensic neuropathology
KW  - forensic neurotraumatology
KW  - immunohistochemistry
KW  - immunocytochemistry
KW  - biomarker
Y1  - 2022
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-325009
VL  - 136
IS  - 6
ER  -