TY  - JOUR
A1  - Enigk, Fabian
A1  - Wagner, Antje
A1  - Samapati, Rudi
A1  - Rittner, Heike
A1  - Brack, Alexander
A1  - Mousa, Shaaban A.
A1  - Schäfer, Michael
A1  - Habazettl, Helmut
A1  - Schäper, Jörn
T1  - Thoracic epidural anesthesia decreases endotoxin-induced endothelial injury
JF  - BMC Anesthesiology
N2  - Background: The sympathetic nervous system is considered to modulate the endotoxin-induced activation of immune cells. Here we investigate whether thoracic epidural anesthesia with its regional symapathetic blocking effect alters endotoxin-induced leukocyte-endothelium activation and interaction with subsequent endothelial injury. Methods: Sprague Dawley rats were anesthetized, cannulated and hemodynamically monitored. E. coli lipopolysaccharide (Serotype 0127: B8, 1.5 mg x kg(-1) x h(-1)) or isotonic saline (controls) was infused for 300 minutes. An epidural catheter was inserted for continuous application of lidocaine or normal saline in endotoxemic animals and saline in controls. After 300 minutes we measured catecholamine and cytokine plasma concentrations, adhesion molecule expression, leukocyte adhesion, and intestinal tissue edema. Results: In endotoxemic animals with epidural saline, LPS significantly increased the interleukin-1 beta plasma concentration (48%), the expression of endothelial adhesion molecules E-selectin (34%) and ICAM-1 (42%), and the number of adherent leukocytes (40%) with an increase in intestinal myeloperoxidase activity (26%) and tissue edema (75%) when compared to healthy controls. In endotoxemic animals with epidural infusion of lidocaine the values were similar to those in control animals, while epinephrine plasma concentration was 32% lower compared to endotoxemic animals with epidural saline. Conclusions: Thoracic epidural anesthesia attenuated the endotoxin-induced increase of IL-1 beta concentration, adhesion molecule expression and leukocyte-adhesion with subsequent endothelial injury. A potential mechanism is the reduction in the plasma concentration of epinephrine.
KW  - endotoxemia
KW  - myeloperoxidase
KW  - endothelial injury
KW  - adhesion molecules
KW  - inflammatory response
KW  - intestinal microvascular perfusion
KW  - cell-adhesion
KW  - induced impairment
KW  - reperfusion injury
KW  - sepsis
KW  - neutrophil
KW  - lidocaine
KW  - lung injury
KW  - cytokines
KW  - epidural anesthesia
Y1  - 2014
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-116787
VL  - 14
IS  - 23
ER  - 
TY  - CHAP
A1  - Clarke, Andrew H.
A1  - Ellgring, Johann Heinrich
A1  - Wagner, Helmut
T1  - Analyse des Blickverhaltens in sozialen Situationen
N2  - Der vorliegende Beitrag behandelt inhaltliche und methodische Probleme einer Längsschnittstudie, die derzeit am MPI für Psychiatrie in München an depressiven Patienten durchgeführt wird. Diese Untersuchung hat einen diagnostischen und einen kommunikationstheoretischen Aspekt. Von diagnostischem Interesse sind die depressionsspezifischen Veränderungen des kommunikativen Verhaltens. Kommunikationstheoretisch gewendet lautet die Frage, wie sich der interne Zustand einer Person in ihrem Verhalten reflektiert. In diesem Beitrag wird die Fragestellung konzentriert auf tonische Eigenschaften des Blickverhaltens im Interaktionsprozeß und deren Stabilität im Krankheitsverlauf. Zunächst wird die theoretische Grundlage referiert, die wir für das Blickverhalten entwickelt haben. Im zweiten Abschnitt wird auf Datenerhebung und Reliabilitätskontrolle eingegangen. Die Struktur der Daten bestimmt die Auswertungsmethoden. Einige der Methoden, mit denen wir derzeit arbeiten, werden im Zusammenhang mit umschriebenen Problemstellungen erläutert: Deskription und Interpretation des tonischen Blickverhaltens depressiver Patienten im Krankheitsverlauf (Abschnitt 3). Zusammenhang der individuellen Blickniveaus von Interaktionspartnern (Abschnitt 4) Deskription eines Dialogs (Interviews) als Sequenz von distinkten Zuständen. In den Zuständen werden Sprech- und Blickverhalten beider Interaktionspartner miteinander kombiniert (Abschnitt 5) Analyse der intraindividuellen Wechselwirkung zwischen Blick- und Sprechverhalten (Abschnitt 6). Die Abschnitte 3 mit 6 werden durch vorläufige Ergebnisse illustriert.
Y1  - 1981
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-43012
ER  - 
TY  - JOUR
A1  - Ellgring, Johann Heinrich
A1  - Wagner, Helmut
A1  - Clarke, Andrew H.
T1  - Binäre Kodierung von Sprechen und Blicken: Validität, Reliabilität und ihre Abhängigkeit von der zeitlichen Auflösung
N2  - Validität und Reliabilität der Sprechkodierung einer Beobachtergruppe wurden in Abhängigkeit von der zeitlichen Auflösung untersucht. Die Validität wurde anhand der Übereinstimmung der Beobachter mit einem automatischen Sprachdetektor berechnet. Die Reliabilitätswerte für die Kodierung von Sprechen und Blikken ergaben sich aus der Übereinstimmung der Beobachter untereinander. Im wesentlichen zeigten sich folgende Ergebnisse: 1. Die Validität/Reliabilität der Sprechkodierung ist eine monotone, nichtlineare Funktion der gewählten Auflösung. Die systematischen Fehler, die auf Latenz und Trägheit der menschlichen Beobachter zurückgehen, werden bei einer Auflösung von 400 msec nahezu vollständig unterdrückt. 2. Weder bei der Erfassung des Sprech- noch des Blickverhaltens lassen sich Anzeichen für Observer-Drift feststellen. Trainierte und untrainierte Beobachter unterscheiden sich nicht signifikant. 3. Die Kodierung des Sprechverhaltens ist geringfügig reliabler als die des Blickverhaltens. Dieser Unterschied kann in der Praxis vernachlässigt werden.
N2  - The reliability of the binary coding of speech and gaze was examined as a function of the temporal resolution of measurement. The reliability was calculated as the agreement between human observers. In addition, the validity of the speech coding could be determined from the agreement between the human observers and an electronic speech detector.
Y1  - 1980
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-43163
ER  - 
TY  - JOUR
A1  - Diers, Johannes
A1  - Acar, Laura
A1  - Wagner, Johanna C.
A1  - Baum, Philip
A1  - Hankir, Mohammed
A1  - Flemming, Sven
A1  - Kastner, Carolin
A1  - Germer, Christoph-Thomas
A1  - L’hoest, Helmut
A1  - Marschall, Ursula
A1  - Lock, Johan Friso
A1  - Wiegering, Armin
T1  - Cancer diagnosis is one quarter lower than the expected cancer incidence in the first year of COVID-19 pandemic in Germany: A retrospective register-based cohort study
JF  - Cancer Communications
N2  - No abstract available.
KW  - cancer diagnosis
KW  - COVID-19 pandemic
KW  - Germany
Y1  - 2022
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-312862
VL  - 42
IS  - 7
ER  - 
TY  - JOUR
A1  - Meier, Johannes P.
A1  - Möbus, Selina
A1  - Heigl, Florian
A1  - Asbach-Nitzsche, Alexandra
A1  - Niller, Hans Helmut
A1  - Plentz, Annelie
A1  - Avsar, Korkut
A1  - Heiß-Neumann, Marion
A1  - Schaaf, Bernhard
A1  - Cassens, Uwe
A1  - Seese, Bernd
A1  - Teschner, Daniel
A1  - Handzhiev, Sabin
A1  - Graf, Uwe
A1  - Lübbert, Christoph
A1  - Steinmaurer, Monika
A1  - Kontogianni, Konstantina
A1  - Berg, Christoph
A1  - Maieron, Andreas
A1  - Blaas, Stefan H.
A1  - Wagner, Ralf
A1  - Deml, Ludwig
A1  - Barabas, Sascha
T1  - Performance of T-Track\(^®\) TB, a novel dual marker RT-qPCR-based whole-blood test for improved detection of active tuberculosis
JF  - Diagnostics
N2  - Tuberculosis (TB) is one of the leading causes of death by an infectious disease. It remains a major health burden worldwide, in part due to misdiagnosis. Therefore, improved diagnostic tests allowing the faster and more reliable diagnosis of patients with active TB are urgently needed. This prospective study examined the performance of the new molecular whole-blood test T-Track\(^®\) TB, which relies on the combined evaluation of IFNG and CXCL10 mRNA levels, and compared it to that of the QuantiFERON\(^®\)-TB Gold Plus (QFT-Plus) enzyme-linked immunosorbent assay (ELISA). Diagnostic accuracy and agreement analyses were conducted on the whole blood of 181 active TB patients and 163 non-TB controls. T-Track\(^®\) TB presented sensitivity of 94.9% and specificity of 93.8% for the detection of active TB vs. non-TB controls. In comparison, the QFT-Plus ELISA showed sensitivity of 84.3%. The sensitivity of T-Track\(^®\) TB was significantly higher (p < 0.001) than that of QFT-Plus. The overall agreement of T-Track\(^®\) TB with QFT-Plus to diagnose active TB was 87.9%. Out of 21 samples with discordant results, 19 were correctly classified by T-Track\(^®\) TB while misclassified by QFT-Plus (T-Track\(^®\) TB-positive/QFT-Plus-negative), and two samples were misclassified by T-Track\(^®\) TB while correctly classified by QFT-Plus (T-Track\(^®\) TB-negative/QFT-Plus-positive). Our results demonstrate the excellent performance of the T-Track\(^®\) TB molecular assay and its suitability to accurately detect TB infection and discriminate active TB patients from non-infected controls.
KW  - tuberculosis
KW  - TB
KW  - active TB
KW  - infection detection
KW  - T-Track\(^®\) TB
KW  - QuantiFERON\(^®\)-TB Gold Plus
KW  - mRNA
KW  - RT-qPCR
KW  - CXCL10
KW  - IFNG
Y1  - 2023
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-304113
SN  - 2075-4418
VL  - 13
IS  - 4
ER  - 
TY  - JOUR
A1  - El-Helou, Sabine M.
A1  - Biegner, Anika-Kerstin
A1  - Bode, Sebastian
A1  - Ehl, Stephan R.
A1  - Heeg, Maximilian
A1  - Maccari, Maria E.
A1  - Ritterbusch, Henrike
A1  - Speckmann, Carsten
A1  - Rusch, Stephan
A1  - Scheible, Raphael
A1  - Warnatz, Klaus
A1  - Atschekzei, Faranaz
A1  - Beider, Renata
A1  - Ernst, Diana
A1  - Gerschmann, Stev
A1  - Jablonka, Alexandra
A1  - Mielke, Gudrun
A1  - Schmidt, Reinhold E.
A1  - Schürmann, Gesine
A1  - Sogkas, Georgios
A1  - Baumann, Ulrich H.
A1  - Klemann, Christian
A1  - Viemann, Dorothee
A1  - Bernuth, Horst von
A1  - Krüger, Renate
A1  - Hanitsch, Leif G.
A1  - Scheibenbogen, Carmen M.
A1  - Wittke, Kirsten
A1  - Albert, Michael H.
A1  - Eichinger, Anna
A1  - Hauck, Fabian
A1  - Klein, Christoph
A1  - Rack-Hoch, Anita
A1  - Sollinger, Franz M.
A1  - Avila, Anne
A1  - Borte, Michael
A1  - Borte, Stephan
A1  - Fasshauer, Maria
A1  - Hauenherm, Anja
A1  - Kellner, Nils
A1  - Müller, Anna H.
A1  - Ülzen, Anett
A1  - Bader, Peter
A1  - Bakhtiar, Shahrzad
A1  - Lee, Jae-Yun
A1  - Heß, Ursula
A1  - Schubert, Ralf
A1  - Wölke, Sandra
A1  - Zielen, Stefan
A1  - Ghosh, Sujal
A1  - Laws, Hans-Juergen
A1  - Neubert, Jennifer
A1  - Oommen, Prasad T.
A1  - Hönig, Manfred
A1  - Schulz, Ansgar
A1  - Steinmann, Sandra
A1  - Klaus, Schwarz
A1  - Dückers, Gregor
A1  - Lamers, Beate
A1  - Langemeyer, Vanessa
A1  - Niehues, Tim
A1  - Shai, Sonu
A1  - Graf, Dagmar
A1  - Müglich, Carmen
A1  - Schmalzing, Marc T.
A1  - Schwaneck, Eva C.
A1  - Tony, Hans-Peter
A1  - Dirks, Johannes
A1  - Haase, Gabriele
A1  - Liese, Johannes G.
A1  - Morbach, Henner
A1  - Foell, Dirk
A1  - Hellige, Antje
A1  - Wittkowski, Helmut
A1  - Masjosthusmann, Katja
A1  - Mohr, Michael
A1  - Geberzahn, Linda
A1  - Hedrich, Christian M.
A1  - Müller, Christiane
A1  - Rösen-Wolff, Angela
A1  - Roesler, Joachim
A1  - Zimmermann, Antje
A1  - Behrends, Uta
A1  - Rieber, Nikolaus
A1  - Schauer, Uwe
A1  - Handgretinger, Rupert
A1  - Holzer, Ursula
A1  - Henes, Jörg
A1  - Kanz, Lothar
A1  - Boesecke, Christoph
A1  - Rockstroh, Jürgen K.
A1  - Schwarze-Zander, Carolynne
A1  - Wasmuth, Jan-Christian
A1  - Dilloo, Dagmar
A1  - Hülsmann, Brigitte
A1  - Schönberger, Stefan
A1  - Schreiber, Stefan
A1  - Zeuner, Rainald
A1  - Ankermann, Tobias
A1  - Bismarck, Philipp von
A1  - Huppertz, Hans-Iko
A1  - Kaiser-Labusch, Petra
A1  - Greil, Johann
A1  - Jakoby, Donate
A1  - Kulozik, Andreas E.
A1  - Metzler, Markus
A1  - Naumann-Bartsch, Nora
A1  - Sobik, Bettina
A1  - Graf, Norbert
A1  - Heine, Sabine
A1  - Kobbe, Robin
A1  - Lehmberg, Kai
A1  - Müller, Ingo
A1  - Herrmann, Friedrich
A1  - Horneff, Gerd
A1  - Klein, Ariane
A1  - Peitz, Joachim
A1  - Schmidt, Nadine
A1  - Bielack, Stefan
A1  - Groß-Wieltsch, Ute
A1  - Classen, Carl F.
A1  - Klasen, Jessica
A1  - Deutz, Peter
A1  - Kamitz, Dirk
A1  - Lassy, Lisa
A1  - Tenbrock, Klaus
A1  - Wagner, Norbert
A1  - Bernbeck, Benedikt
A1  - Brummel, Bastian
A1  - Lara-Villacanas, Eusebia
A1  - Münstermann, Esther
A1  - Schneider, Dominik T.
A1  - Tietsch, Nadine
A1  - Westkemper, Marco
A1  - Weiß, Michael
A1  - Kramm, Christof
A1  - Kühnle, Ingrid
A1  - Kullmann, Silke
A1  - Girschick, Hermann
A1  - Specker, Christof
A1  - Vinnemeier-Laubenthal, Elisabeth
A1  - Haenicke, Henriette
A1  - Schulz, Claudia
A1  - Schweigerer, Lothar
A1  - Müller, Thomas G.
A1  - Stiefel, Martina
A1  - Belohradsky, Bernd H.
A1  - Soetedjo, Veronika
A1  - Kindle, Gerhard
A1  - Grimbacher, Bodo
T1  - The German national registry of primary immunodeficiencies (2012-2017)
JF  - Frontiers in Immunology
N2  - Introduction: The German PID-NET registry was founded in 2009, serving as the first national registry of patients with primary immunodeficiencies (PID) in Germany. It is part of the European Society for Immunodeficiencies (ESID) registry. The primary purpose of the registry is to gather data on the epidemiology, diagnostic delay, diagnosis, and treatment of PIDs. 

Methods: Clinical and laboratory data was collected from 2,453 patients from 36 German PID centres in an online registry. Data was analysed with the software Stata® and Excel. 

Results: The minimum prevalence of PID in Germany is 2.72 per 100,000 inhabitants. Among patients aged 1-25, there was a clear predominance of males. The median age of living patients ranged between 7 and 40 years, depending on the respective PID. Predominantly antibody disorders were the most prevalent group with 57% of all 2,453 PID patients (including 728 CVID patients). A gene defect was identified in 36% of patients. Familial cases were observed in 21% of patients. The age of onset for presenting symptoms ranged from birth to late adulthood (range 0-88 years). Presenting symptoms comprised infections (74%) and immune dysregulation (22%). Ninety-three patients were diagnosed without prior clinical symptoms. Regarding the general and clinical diagnostic delay, no PID had undergone a slight decrease within the last decade. However, both, SCID and hyper IgE-syndrome showed a substantial improvement in shortening the time between onset of symptoms and genetic diagnosis. Regarding treatment, 49% of all patients received immunoglobulin G (IgG) substitution (70%-subcutaneous; 29%-intravenous; 1%-unknown). Three-hundred patients underwent at least one hematopoietic stem cell transplantation (HSCT). Five patients had gene therapy. 

Conclusion: The German PID-NET registry is a precious tool for physicians, researchers, the pharmaceutical industry, politicians, and ultimately the patients, for whom the outcomes will eventually lead to a more timely diagnosis and better treatment.
KW  - registry for primary immunodeficiency
KW  - primary immunodeficiency (PID)
KW  - German PID-NET registry
KW  - PID prevalence
KW  - European Society for Immunodeficiencies (ESID)
KW  - IgG substitution therapy
KW  - CVID
Y1  - 2019
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-226629
VL  - 10
ER  -