TY  - JOUR
A1  - Kurabi, Arwa
A1  - Schaerer, Daniel
A1  - Noack, Volker
A1  - Bernhardt, Marlen
A1  - Pak, Kwang
A1  - Alexander, Thomas
A1  - Husseman, Jacob
A1  - Nguyen, Quyen
A1  - Harris, Jeffrey P.
A1  - Ryan, Allen F.
T1  - Active Transport of Peptides Across the Intact Human Tympanic Membrane
JF  - Scientific Reports
N2  - We previously identified peptides that are actively transported across the intact tympanic membrane (TM) of rats with infected middle ears. To assess the possibility that this transport would also occur across the human TM, we first developed and validated an assay to evaluate transport in vitro using fragments of the TM. Using this assay, we demonstrated the ability of phage bearing a TM-transiting peptide to cross freshly dissected TM fragments from infected rats or from uninfected rats, guinea pigs and rabbits. We then evaluated transport across fragments of the human TM that were discarded during otologic surgery. Human trans-TM transport was similar to that seen in the animal species. Finally, we found that free peptide, unconnected to phage, was transported across the TM at a rate comparable to that seen for peptide-bearing phage. These studies provide evidence supporting the concept of peptide-mediated drug delivery across the intact TM and into the middle ears of patients.
KW  - assay systems
KW  - biological models
Y1  - 2018
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-230929
VL  - 8
ER  -