TY  - JOUR
A1  - Song, Ning-Ning
A1  - Xiu, Jian-Bo
A1  - Huang, Ying
A1  - Chen, Jia-Yin
A1  - Zhang, Lei
A1  - Gutknecht, Lise
A1  - Lesch, Klaus Peter
A1  - Li, He
A1  - Ding, Yu-Qiang
T1  - Adult Raphe-Specific Deletion of Lmx1b Leads to Central Serotonin Deficiency
JF  - PLoS ONE
N2  - The transcription factor Lmx1b is essential for the differentiation and survival of central serotonergic (5-HTergic) neurons during embryonic development. However, the role of Lmx1b in adult 5-HTergic neurons is unknown. We used an inducible Cre-LoxP system to selectively inactivate Lmx1b expression in the raphe nuclei of adult mice. Pet1-CreER(T2) mice were generated and crossed with Lmx1b(flox/flox) mice to obtain Pet1-CreER(T2); Lmx1b(flox/flox) mice (which termed as Lmx1b iCKO). After administration of tamoxifen, the level of 5-HT in the brain of Lmx1b iCKO mice was reduced to 60% of that in control mice, and the expression of tryptophan hydroxylase 2 (Tph2), serotonin transporter (Sert) and vesicular monoamine transporter 2 (Vmat2) was greatly down-regulated. On the other hand, the expression of dopamine and norepinephrine as well as aromatic L-amino acid decarboxylase (Aadc) and Pet1 was unchanged. Our results reveal that Lmx1b is required for the biosynthesis of 5-HT in adult mouse brain, and it may be involved in maintaining normal functions of central 5-HTergic neurons by regulating the expression of Tph2, Sert and Vmat2.
KW  - Molecular-genetics
KW  - Sonic hedgehog
KW  - Neurons
KW  - Mice
KW  - Brain
KW  - Expression
KW  - System
KW  - PET-1
KW  - Transporter
KW  - Disorders
Y1  - 2011
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-133581
VL  - 6
IS  - 1
ER  - 
TY  - JOUR
A1  - Liu, Han
A1  - Chen, Chunhai
A1  - Gao, Zexia
A1  - Min, Jiumeng
A1  - Gu, Yongming
A1  - Jian, Jianbo
A1  - Jiang, Xiewu
A1  - Cai, Huimin
A1  - Ebersberger, Ingo
A1  - Xu, Meng
A1  - Zhang, Xinhui
A1  - Chen, Jianwei
A1  - Luo, Wei
A1  - Chen, Boxiang
A1  - Chen, Junhui
A1  - Liu, Hong
A1  - Li, Jiang
A1  - Lai, Ruifang
A1  - Bai, Mingzhou
A1  - Wei, Jin
A1  - Yi, Shaokui
A1  - Wang, Huanling
A1  - Cao, Xiaojuan
A1  - Zhou, Xiaoyun
A1  - Zhao, Yuhua
A1  - Wei, Kaijian
A1  - Yang, Ruibin
A1  - Liu, Bingnan
A1  - Zhao, Shancen
A1  - Fang, Xiaodong
A1  - Schartl, Manfred
A1  - Qian, Xueqiao
A1  - Wang, Weimin
T1  - The draft genome of blunt snout bream (Megalobrama amblycephala) reveals the development of intermuscular bone and adaptation to herbivorous diet
JF  - GigaScience
N2  - The blunt snout bream Megalobrama amblycephala is the economically most important cyprinid fish species. As an herbivore, it can be grown by eco-friendly and resource-conserving aquaculture. However, the large number of intermuscular bones in the trunk musculature is adverse to fish meat processing and consumption. As a first towards optimizing this aquatic livestock, we present a 1.116-Gb draft genome of M. amblycephala, with 779.54 Mb anchored on 24 linkage groups. Integrating spatiotemporal transcriptome analyses, we show that intermuscular bone is formed in the more basal teleosts by intramembranous ossification and may be involved in muscle contractibility and coordinating cellular events. Comparative analysis revealed that olfactory receptor genes, especially of the beta type, underwent an extensive expansion in herbivorous cyprinids, whereas the gene for the umami receptor T1R1 was specifically lost in M. amblycephala. The composition of gut microflora, which contributes to the herbivorous adaptation of M. amblycephala, was found to be similar to that of other herbivores. As a valuable resource for the improvement of M. amblycephala livestock, the draft genome sequence offers new insights into the development of intermuscular bone and herbivorous adaptation.
KW  - Megalobrama amblycephala
KW  - whole genome
KW  - herbivorous diet
KW  - intermuscular bone
KW  - transcriptome
KW  - gut microflora
Y1  - 2017
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-170844
VL  - 6
IS  - 7
ER  -