TY  - JOUR
A1  - Suliman, Salwa
A1  - Sun, Yang
A1  - Pedersen, Torbjorn O.
A1  - Xue, Ying
A1  - Nickel, Joachim
A1  - Waag, Thilo
A1  - Finne-Wistrand, Anna
A1  - Steinmüller-Nethl, Doris
A1  - Krueger, Anke
A1  - Costea, Daniela E.
A1  - Mustafa, Kamal
T1  - In vivo host response and degradation of copolymer scaffolds functionalized with nanodiamonds and bone morphogenetic protein 2
JF  - Advanced Healthcare Materials
N2  - The aim is to evaluate the effect of modifying poly[(L-lactide)-co-(epsilon-caprolactone)] scaffolds (PLCL) with nanodiamonds (nDP) or with nDP+physisorbed BMP-2 (nDP+BMP-2) on in vivo host tissue response and degradation. The scaffolds are implanted subcutaneously in Balb/c mice and retrieved after 1, 8, and 27 weeks. Molecular weight analysis shows that modified scaffolds degrade faster than the unmodified. Gene analysis at week 1 shows highest expression of proinflammatory markers around nDP scaffolds; although the presence of inflammatory cells and foreign body giant cells is more prominent around the PLCL. Tissue regeneration markers are highly expressed in the nDP+BMP-2 scaffolds at week 8. A fibrous capsule is detectable by week 8, thinnest around nDP scaffolds and at week 27 thickest around PLCL scaffolds. mRNA levels of ALP, COL1 alpha 2, and ANGPT1 are signifi cantly upregulating in the nDP+BMP-2 scaffolds at week 1 with ectopic bone seen at week 8. Even when almost 90% of the scaffold is degraded at week 27, nDP are observable at implantation areas without adverse effects. In conclusion, modifying PLCL scaffolds with nDP does not aggravate the host response and physisorbed BMP-2 delivery attenuates infl ammation while lowering the dose of BMP-2 to a relatively safe and economical level.
KW  - inflammatory response
KW  - biocompatibility
KW  - BMP-2 delivery
KW  - inflammation
KW  - tissue engineering
Y1  - 2016
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-189764
VL  - 5
IS  - 6
ER  - 
TY  - JOUR
A1  - Xu, Jietao
A1  - Fahmy-Garcia, Shorouk
A1  - Wesdorp, Marinus A.
A1  - Kops, Nicole
A1  - Forte, Lucia
A1  - De Luca, Claudio
A1  - Misciagna, Massimiliano Maraglino
A1  - Dolcini, Laura
A1  - Filardo, Giuseppe
A1  - Labberté, Margot
A1  - Vancíková, Karin
A1  - Kok, Joeri
A1  - van Rietbergen, Bert
A1  - Nickel, Joachim
A1  - Farrell, Eric
A1  - Brama, Pieter A. J.
A1  - van Osch, Gerjo J. V. M.
T1  - Effectiveness of BMP-2 and PDGF-BB adsorption onto a collagen/collagen-magnesium-hydroxyapatite scaffold in weight-bearing and non-weight-bearing osteochondral defect bone repair: in vitro, ex vivo and in vivo evaluation
JF  - Journal of Functional Biomaterials
N2  - Despite promising clinical results in osteochondral defect repair, a recently developed bi-layered collagen/collagen-magnesium-hydroxyapatite scaffold has demonstrated less optimal subchondral bone repair. This study aimed to improve the bone repair potential of this scaffold by adsorbing bone morphogenetic protein 2 (BMP-2) and/or platelet-derived growth factor-BB (PDGF-BB) onto said scaffold. The in vitro release kinetics of BMP-2/PDGF-BB demonstrated that PDGF-BB was burst released from the collagen-only layer, whereas BMP-2 was largely retained in both layers. Cell ingrowth was enhanced by BMP-2/PDFG-BB in a bovine osteochondral defect ex vivo model. In an in vivo semi-orthotopic athymic mouse model, adding BMP-2 or PDGF-BB increased tissue repair after four weeks. After eight weeks, most defects were filled with bone tissue. To further investigate the promising effect of BMP-2, a caprine bilateral stifle osteochondral defect model was used where defects were created in weight-bearing femoral condyle and non-weight-bearing trochlear groove locations. After six months, the adsorption of BMP-2 resulted in significantly less bone repair compared with scaffold-only in the femoral condyle defects and a trend to more bone repair in the trochlear groove. Overall, the adsorption of BMP-2 onto a Col/Col-Mg-HAp scaffold reduced bone formation in weight-bearing osteochondral defects, but not in non-weight-bearing osteochondral defects.
KW  - tissue engineering
KW  - regenerative medicine
KW  - osteochondral lesion
KW  - biocompatible materials
KW  - bone morphogenetic proteins
KW  - platelet-derived growth factor
KW  - animal model
KW  - weight-bearing
Y1  - 2023
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-304019
SN  - 2079-4983
VL  - 14
IS  - 2
ER  -