TY  - JOUR
A1  - Kann, Simone
A1  - Blessmann, Joerg
A1  - Winkelmann, Yvonne
A1  - Hansen, Jessica
A1  - Maya Amaya, Leonardo J.
A1  - Rivera Salcedo, Gadith E.
A1  - Halas, Hussein El
A1  - Schmidt‐Chanasit, Jonas
A1  - Keoviengkhone, Latdamone
A1  - Sopraseuth, Vatsana
A1  - Deschermeier, Christina
A1  - Mika, Angela
T1  - Dengue virus detection in Lao PDR and Colombia: Comparative evaluation of PCR tests
JF  - Tropical Medicine & International Health
N2  - Objectives
Dengue virus (DENV) detection by polymerase chain reaction (PCR) facilitates diagnosis of dengue fever, which is the most frequent arboviral disease globally. Two studies were performed in countries with high dengue incidence, to assess the diagnostic performance of different PCR techniques.
Methods/Results
Two hundred and seventy‐nine acute phase blood samples from febrile patients were analyzed for DENV by the RealStar Dengue RT‐PCR kit (Altona Diagnostics) as gold standard in comparison with the Tropical Fever Core multiplex PCR (Fast Track Diagnostics). In total, 102 samples collected in Savannakhet Province (Lao PDR, Southeast Asia) in 2013 and 35 samples from Valledupar (Colombia, South America) tested positive for DENV by RealStar RT‐PCR. In comparison, the Tropical Fever Core multiplex PCR detected 65.0% (65/102) and 68.6% (24/35) of these samples as positive for DENV in Savannakhet and Valledupar, respectively. Diagnostic sensitivity of the multiplex PCR strongly correlated with viral load. A subset of DENV PCR‐confirmed samples was additionally tested by BNITM in house Dengue Type RT‐PCR in comparison with two commercial test kits (RealStar Dengue Type RT‐PCR [Altona Diagnostics], Dengue differentiation PCR [Fast Track Diagnostics]). The leading dengue serotype in Savannakhet was DENV‐3 (58% [29/50]), while DENV‐1 (53.8% [14/26]) was the predominant serotype found in samples collected in Valledupar by BNITM‐type PCR. However, three DENV serotypes were circulating in Valledupar and in Savannakhet. In 2015, additional studies found predominantly DENV‐4 (71% [12/17]) in Savannakhet.
Conclusions
Both studies emphasized that routine diagnostics in both regions will benefit from an expanded use of highly sensitive pan‐dengue PCRs.
KW  - Colombia
KW  - dengue infections
KW  - dengue serotypes
KW  - Lao PDR
KW  - PCR diagnostics
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-262703
VL  - 26
IS  - 10
SP  - 1296
EP  - 1302
ER  -