TY  - THES
A1  - Ramirez Pineda, José Robinson
T1  - Dendritic cells activated by CpG motifs are potent inducers of a Th1 immune response that protects mice against leishmaniasis
T1  - CpG-aktivierte dendritische-Zellen induzieren eine Th1 immunantwort die Mäuse gegen Leishmaniose schütz
N2  - The present investigation report a protocol to obtain dendritic cells (DC) that protects mice against fatal leishmaniasis. DC were generated from bone marrow precursors, pulsed with leishmanial antigen and activated with CpG oligodeoxinucleotides. Mice that were vaccinated with these cells were strongly protected against the clinical and parasitological manifestations of leishmaniasis and developed a Th1 immune response. protection was solid and long-lasting, and was also dependent of the via of administration. Whe the mechanism of protection was studied, it was observed that the availability of the cytokine interleukin-12 at the time of vaccination was a key requirement, but that the source of this cytokine is not the donor cells but unidentified cells from the recipients.
N2  - En esta tesis se reporta un prtocolo para obtener celulas dendriticas (CD) que inducen una respuesta protectora contra la leishmaniasis en ratones susceptibles. Las CD se generaron de precursores de medula osea, se pulsaron con antigeno de Leishmania y se activaron con oligonucleotidos que contienen motivos CpG. Cuando los ratones se vacunan con estas celulas se observa una fuerte proteccion clinica y parasitologica contra la leishmaniasis. Los ratones se protegen debido a que desarrollan una respuesta inmune de tipo Th1, en contraste con la respuesta Th2 desarrollada por los ratones control. La proteccion fue solida y duradera y fue dependiente de la via de administracion de las CD. Cuando se estudio el mecanismo de proteccion, se encontro que se requiere la presencia de la citokina interleukina 12 en el momento de la vacunacion, y que la fuente de esta citokina no son las celulas donadoras, sino celulas de los ratones recipientes.
KW  - Leishmaniose
KW  - Dendritische Zelle
KW  - Immunreaktion
KW  - Dendrtitischer Zellen
KW  - Th1 immunantwort
KW  - leishmaniose
KW  - CpG
KW  - Dendritic cells
KW  - Th1 response
KW  - leishmaniasis
KW  - CpG
Y1  - 2003
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-8410
ER  - 
TY  - JOUR
A1  - Gutiérrez, Gustavo
A1  - Giraldo-Dávila, Deisy
A1  - Combariza, Marianny Y.
A1  - Holzgrabe, Ulrike
A1  - Tabares-Guevara, Jorge Humberto
A1  - Ramírez-Pineda, José Robinson
A1  - Acín, Sergio
A1  - Muñoz, Diana Lorena
A1  - Montoya, Guillermo
A1  - Balcazar, Norman
T1  - Serjanic acid improves immunometabolic markers in a diet-induced obesity mouse model
JF  - Molecules
N2  - Plant extracts from Cecropia genus have been used by Latin-American traditional medicine to treat metabolic disorders and diabetes. Previous reports have shown that roots of Cecropia telenitida that contains serjanic acid as one of the most prominent and representative pentacyclic triterpenes. The study aimed to isolate serjanic acid and evaluate its effect in a prediabetic murine model by oral administration. A semi-pilot scale extraction was established and serjanic acid purification was followed using direct MALDI-TOF analysis. A diet induced obesity mouse model was used to determine the impact of serjanic acid over selected immunometabolic markers. Mice treated with serjanic acid showed decreased levels of cholesterol and triacylglycerols, increased blood insulin levels, decreased fasting blood glucose and improved glucose tolerance, and insulin sensitivity. At transcriptional level, the reduction of inflammation markers related to adipocyte differentiation is reported.
KW  - Cecropia telenitida
KW  - serjanic acid
KW  - type 2 diabetes
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-203253
SN  - 1420-3049
VL  - 25
IS  - 7
ER  -