TY  - JOUR
A1  - Berger, Susanne
A1  - Ellersiek, Ulrike
A1  - Steinmüller, Klaus
T1  - Cyanobacteria contain a mitochrondrial complex I-homologous NADH-dehydrogenase
N2  - Thylakoid and cytoplasmic membranes of the cyanobacterium Syncchocystis sp. PCC 6803 were purified by sucrose gradient centrifugation. Both membranes oxidize NADH in a rotenone-sensitive reaction. Antibodies prepared against psbG/ndhKand ndhJ fusion proteins detect the corresponding polypeptides in both membrane preparations. This demonstrates that a NADH-dehydrogenase, homologous to the mitochondrial NADHubiquinone-oxidoreductase (complex I of the respiratory chain) is present in cyanobacteria, The NADH-dehydrogenase can be solubilized with the detergent /-D-dodecylmaltoside. Sedimentation analysis of the solubilized enzyme on a sucrose gradient indicates that it is a multisubunit protein complex.
KW  - Respiratory chain
KW  - NADH-dehydrogenase
KW  - Cyanobacteria
KW  - Synechocystis 6803
Y1  - 1991
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-31223
ER  -