TY  - JOUR
A1  - Bohnert, Simone
A1  - Georgiades, Kosmas
A1  - Monoranu, Camelia-Maria
A1  - Bohnert, Michael
A1  - Büttner, Andreas
A1  - Ondruschka, Benjamin
T1  - Quantitative evidence of suppressed TMEM119 microglial immunohistochemistry in fatal morphine intoxications
JF  - International Journal of Legal Medicine
N2  - The aim of this pilot study was to investigate the diagnostic potential of TMEM119 as a useful microglia-specific marker in combination with immunostainings for phagocytic function and infiltrating capacity of monocytes in cases of lethal monosubstance intoxications by morphine (MOR), methamphetamine (METH), and of ethanol-associated death (ETH) respectively. Human brain tissue samples were obtained from forensic autopsies of cases with single substance abuse (MOR, n = 8; ETH, n = 10; METH, n = 9) and then compared to a cohort of cardiovascular fatalities as controls (n = 9). Brain tissue samples of cortex, white matter, and hippocampus were collected and stained immunohistochemically with antibodies against TMEM119, CD68KiM1P, and CCR2. We could document the lowest density of TMEM119-positive cells in MOR deaths with highly significant differences to the control densities in all three regions investigated. In ETH and METH deaths, the expression of TMEM119 was comparable to cell densities in controls. The results indicate that the immunoreaction in brain tissue is different in these groups depending on the drug type used for abuse.
KW  - immunohistochemistry
KW  - drug abuse
KW  - forensic neuropathology
KW  - neuroinflammation
KW  - neurotoxicity
KW  - microglia
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-266934
SN  - 1437-1596
VL  - 135
IS  - 6
ER  -