TY  - JOUR
A1  - Essig, Fabian
A1  - Babilon, Lilith
A1  - Vollmuth, Christoph
A1  - Kollikowski, Alexander M.
A1  - Pham, Mirko
A1  - Solymosi, László
A1  - Haeusler, Karl Georg
A1  - Kraft, Peter
A1  - Stoll, Guido
A1  - Schuhmann, Michael K.
T1  - High mobility group box 1 protein in cerebral thromboemboli
JF  - International Journal of Molecular Sciences
N2  - High-mobility group box 1 protein (HMGB1) is a damage-associated molecular pattern (DAMP) involved in neutrophil extracellular trap (NET) formation and thrombosis. NETs are regularly found in cerebral thromboemboli. We here analyzed associated HMGB1 expression in human thromboemboli retrieved via mechanical thrombectomy from 37 stroke patients with large vessel occlusion. HMGB1 was detected in all thromboemboli, accounting for 1.7% (IQR 0.6–6.2%) of the total thromboemboli area and was found to be colocalized with neutrophils and NETs and in spatial proximity to platelets. Correlation analysis revealed that the detection of HMGB1 was strongly related to the number of neutrophils (r = 0.58, p = 0.0002) and platelets (r = 0.51, p = 0.001). Our results demonstrate that HMGB1 is a substantial constituent of thromboemboli causing large vessel occlusion stroke.
KW  - acute ischemic stroke
KW  - thromboemboli
KW  - HMGB1
KW  - neutrophils
KW  - platelets
KW  - immunohistochemistry
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-265568
SN  - 1422-0067
VL  - 22
IS  - 20
ER  - 
TY  - JOUR
A1  - Essig, Fabian
A1  - Kollikowski, Alexander M.
A1  - Pham, Mirko
A1  - Solymosi, László
A1  - Stoll, Guido
A1  - Haeusler, Karl Georg
A1  - Kraft, Peter
A1  - Schuhmann, Michael K.
T1  - Immunohistological analysis of neutrophils and neutrophil extracellular traps in human thrombemboli causing acute ischemic stroke
JF  - International Journal of Molecular Sciences
N2  - Ischemic stroke caused by thromboembolic occlusion of large cerebral arteries, such as the internal carotid (ICA) and/or the middle cerebral artery (MCA), is treated by mechanical thrombectomy (MT). MT allows salvage of the vessel-occluding thrombemboli, which most frequently originate from the left atrium or the left ventricle of the heart or from sites of plaque rupture within large arteries above the heart. Clot composition may influence the efficacy of (intravenous) thrombolysis and MT, respectively. We analyzed 37 human thrombemboli obtained from acute ischemic stroke patients during MT with special emphasis on histological staining of neutrophils and neutrophil extracellular traps (NETs). We found neutrophils as the main cellular component of cerebral thrombemboli but encountered considerable morphological heterogeneity. Neutrophils accumulated in the border region of fibrin-rich structures indicating possible interaction of neutrophils with distinct structural thrombembolus components. Web-like NETs were found in 35 of 37 thrombemboli in varying amounts. NETs were almost exclusively found within fibrin-rich areas. Importantly, stroke etiology, age and present oral anticoagulation was associated with morphological patterns and the amount of neutrophils. Correlation of histological data and imaging data revealed that relative Hounsfield units of cerebral thrombemboli positively correlated with the amount of red blood cells. In summary, our results demonstrate that neutrophils and NETs are substantial constituents of cerebral thrombemboli and contribute to their structural complexity.
KW  - acute ischemic stroke
KW  - thrombemboli
KW  - neutrophils
KW  - NETs
KW  - immunohistochemistry
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-236192
SN  - 1422-0067
VL  - 21
IS  - 19
ER  - 
TY  - JOUR
A1  - Solymosi, László
T1  - Expanding the Assessment of Research Activity
JF  - Clinical Neuroradiology
N2  - No abstract available.
KW  - research activity
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-232845
SN  - 1869-1439
VL  - 30
ER  - 
TY  - JOUR
A1  - Solymosi, László
T1  - Three Decades of Success for Clinical Neuroradiology
JF  - Clinical Neuroradiology
N2  - No abstract available.
KW  - Clinical Neuroradiology
KW  - journal
KW  - editorial board
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-264772
VL  - 31
IS  - 3
ER  - 
TY  - JOUR
A1  - Solymosi, László
T1  - Clinical Neuroradiology: challenges and perspectives
JF  - Clinical Neuroradiology
N2  - No abstract available.
KW  - Clinical Neuroradiology
KW  - editorial
Y1  - 2022
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-324537
VL  - 32
IS  - 3
ER  - 
TY  - JOUR
A1  - Solymosi, László
T1  - It’s time to go …
JF  - Clinical Neuroradiology
N2  - No abstract available.
KW  - editorial
Y1  - 2022
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-324997
VL  - 32
IS  - 4
ER  -