TY  - JOUR
A1  - Pauli, Martin
A1  - Paul, Mila M.
A1  - Proppert, Sven
A1  - Mrestani, Achmed
A1  - Sharifi, Marzieh
A1  - Repp, Felix
A1  - Kürzinger, Lydia
A1  - Kollmannsberger, Philip
A1  - Sauer, Markus
A1  - Heckmann, Manfred
A1  - Sirén, Anna-Leena
T1  - Targeted volumetric single-molecule localization microscopy of defined presynaptic structures in brain sections
JF  - Communications Biology
N2  - Revealing the molecular organization of anatomically precisely defined brain regions is necessary for refined understanding of synaptic plasticity. Although three-dimensional (3D) single-molecule localization microscopy can provide the required resolution, imaging more than a few micrometers deep into tissue remains challenging. To quantify presynaptic active zones (AZ) of entire, large, conditional detonator hippocampal mossy fiber (MF) boutons with diameters as large as 10 mu m, we developed a method for targeted volumetric direct stochastic optical reconstruction microscopy (dSTORM). An optimized protocol for fast repeated axial scanning and efficient sequential labeling of the AZ scaffold Bassoon and membrane bound GFP with Alexa Fluor 647 enabled 3D-dSTORM imaging of 25 mu m thick mouse brain sections and assignment of AZs to specific neuronal substructures. Quantitative data analysis revealed large differences in Bassoon cluster size and density for distinct hippocampal regions with largest clusters in MF boutons. Pauli et al. develop targeted volumetric dSTORM in order to image large hippocampal mossy fiber boutons (MFBs) in brain slices. They can identify synaptic targets of individual MFBs and measured size and density of Bassoon clusters within individual untruncated MFBs at nanoscopic resolution.
KW  - mossy fiber synapses
KW  - CA3 pyrimidal cells
KW  - CA2+ channels
KW  - active zone
KW  - hippocampal
KW  - release
KW  - plasticity
KW  - proteins
KW  - platform
KW  - reveals
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-259830
VL  - 4
ER  -