TY  - JOUR
A1  - Pereira, Ana Rita
A1  - Trivanović, Drenka
A1  - Stahlhut, Philipp
A1  - Rudert, Maximilian
A1  - Groll, Jürgen
A1  - Herrmann, Marietta
T1  - Preservation of the naïve features of mesenchymal stromal cells in vitro: Comparison of cell- and bone-derived decellularized extracellular matrix
JF  - Journal of Tissue Engineering
N2  - The fate and behavior of bone marrow mesenchymal stem/stromal cells (BM-MSC) is bidirectionally influenced by their microenvironment, the stem cell niche, where a magnitude of biochemical and physical cues communicate in an extremely orchestrated way. It is known that simplified 2D in vitro systems for BM-MSC culture do not represent their naïve physiological environment. Here, we developed four different 2D cell-based decellularized matrices (dECM) and a 3D decellularized human trabecular-bone scaffold (dBone) to evaluate BM-MSC behavior. The obtained cell-derived matrices provided a reliable tool for cell shape-based analyses of typical features associated with osteogenic differentiation at high-throughput level. On the other hand, exploratory proteomics analysis identified native bone-specific proteins selectively expressed in dBone but not in dECM models. Together with its architectural complexity, the physico-chemical properties of dBone triggered the upregulation of stemness associated genes and niche-related protein expression, proving in vitro conservation of the naïve features of BM-MSC.
KW  - decellularization
KW  - bone model
KW  - stem cell niche
KW  - stemness
KW  - osteogenesis
KW  - 3D models
Y1  - 2022
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-268835
VL  - 13
ER  - 
TY  - JOUR
A1  - Ramírez-Rodríguez, Gloria Belén
A1  - Pereira, Ana Rita
A1  - Herrmann, Marietta
A1  - Hansmann, Jan
A1  - Delgado-López, José Manuel
A1  - Sprio, Simone
A1  - Tampieri, Anna
A1  - Sandri, Monica
T1  - Biomimetic mineralization promotes viability and differentiation of human mesenchymal stem cells in a perfusion bioreactor
JF  - International Journal of Molecular Sciences
N2  - In bone tissue engineering, the design of 3D systems capable of recreating composition, architecture and micromechanical environment of the native extracellular matrix (ECM) is still a challenge. While perfusion bioreactors have been proposed as potential tool to apply biomechanical stimuli, its use has been limited to a low number of biomaterials. In this work, we propose the culture of human mesenchymal stem cells (hMSC) in biomimetic mineralized recombinant collagen scaffolds with a perfusion bioreactor to simultaneously provide biochemical and biophysical cues guiding stem cell fate. The scaffolds were fabricated by mineralization of recombinant collagen in the presence of magnesium (RCP.MgAp). The organic matrix was homogeneously mineralized with apatite nanocrystals, similar in composition to those found in bone. X-Ray microtomography images revealed isotropic porous structure with optimum porosity for cell ingrowth. In fact, an optimal cell repopulation through the entire scaffolds was obtained after 1 day of dynamic seeding in the bioreactor. Remarkably, RCP.MgAp scaffolds exhibited higher cell viability and a clear trend of up-regulation of osteogenic genes than control (non-mineralized) scaffolds. Results demonstrate the potential of the combination of biomimetic mineralization of recombinant collagen in presence of magnesium and dynamic culture of hMSC as a promising strategy to closely mimic bone ECM.
KW  - scaffold
KW  - perfusion bioreactor
KW  - collagen
KW  - apatite nanoparticles
KW  - magnesium
KW  - human mesenchymal stem cell
KW  - osteogenesis
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-285804
SN  - 1422-0067
VL  - 22
IS  - 3
ER  -