TY  - JOUR
A1  - Kasten, Annika
A1  - Naser, Tamara
A1  - Brüllhoff, Kristina
A1  - Fiedler, Jörg
A1  - Müller, Petra
A1  - Möller, Martin
A1  - Rychly, Joachim
A1  - Groll, Jürgen
A1  - Brenner, Rolf E.
T1  - Guidance of Mesenchymal Stem Cells on Fibronectin Structured Hydrogel Films
JF  - PLOS ONE
N2  - Designing of implant surfaces using a suitable ligand for cell adhesion to stimulate specific biological responses of stem cells will boost the application of regenerative implants. For example, materials that facilitate rapid and guided migration of stem cells would promote tissue regeneration. When seeded on fibronectin (FN) that was homogeneously immmobilized to NCO-sP(EO-stat-PO), which otherwise prevents protein binding and cell adhesion, human mesenchymal stem cells (MSC) revealed a faster migration, increased spreading and a more rapid organization of different cellular components for cell adhesion on fibronectin than on a glass surface. To further explore, how a structural organization of FN controls the behavior of MSC, adhesive lines of FN with varying width between 10 mu m and 80 mu m and spacings between 5 mu m and 20 mu m that did not allow cell adhesion were generated. In dependance on both line width and gaps, cells formed adjacent cell contacts, were individually organized in lines, or bridged the lines. With decreasing sizes of FN lines, speed and directionality of cell migration increased, which correlated with organization of the actin cytoskeleton, size and shape of the nuclei as well as of focal adhesions. Together, defined FN lines and gaps enabled a fine tuning of the structural organization of cellular components and migration. Microstructured adhesive substrates can mimic the extracellular matrix in vivo and stimulate cellular mechanisms which play a role in tissue regeneration.
KW  - adhesion dynamics
KW  - migration
KW  - coatings
KW  - force
KW  - networks
KW  - traction
KW  - stress
KW  - tension
KW  - focal adhesions
KW  - tissue morphogenesis
Y1  - 2014
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-114897
VL  - 9
IS  - 10
ER  -