TY - JOUR A1 - Youssif, Khayrya A. A1 - Haggag, Eman G. A1 - Elshamy, Ali M. A1 - Rabeh, Mohamed A. A1 - Gabr, Nagwan M. A1 - Seleem, Amany A1 - Salem, M. Alaraby A1 - Hussein, Ahmed S. A1 - Krischke, Markus A1 - Mueller, Martin J. A1 - Ramadan Abdelmohsen, Usama T1 - Anti-Alzheimer potential, metabolomic profiling and molecular docking of green synthesized silver nanoparticles of Lampranthus coccineus and Malephora lutea aqueous extracts JF - PLoS ONE N2 - The green synthesis of silver nanoparticles (SNPs) using plant extracts is an eco-friendly method. It is a single step and offers several advantages such as time reducing, cost-effective and environmental non-toxic. Silver nanoparticles are a type of Noble metal nanoparticles and it has tremendous applications in the field of diagnostics, therapeutics, antimicrobial activity, anticancer and neurodegenerative diseases. In the present work, the aqueous extracts of aerial parts of Lampranthus coccineus and Malephora lutea F. Aizoaceae were successfully used for the synthesis of silver nanoparticles. The formation of silver nanoparticles was early detected by a color change from pale yellow to reddish-brown color and was further confirmed by transmission electron microscope (TEM), UV–visible spectroscopy, Fourier transform infrared (FTIR) spectroscopy, dynamic light scattering (DLS), X-ray diffraction (XRD), and energy-dispersive X-ray diffraction (EDX). The TEM analysis of showed spherical nanoparticles with a mean size between 12.86 nm and 28.19 nm and the UV- visible spectroscopy showed λ\(_{max}\) of 417 nm, which confirms the presence of nanoparticles. The neuroprotective potential of SNPs was evaluated by assessing the antioxidant and cholinesterase inhibitory activity. Metabolomic profiling was performed on methanolic extracts of L. coccineus and M. lutea and resulted in the identification of 12 compounds, then docking was performed to investigate the possible interaction between the identified compounds and human acetylcholinesterase, butyrylcholinesterase, and glutathione transferase receptor, which are associated with the progress of Alzheimer’s disease. Overall our SNPs highlighted its promising potential in terms of anticholinesterase and antioxidant activity as plant-based anti-Alzheimer drug and against oxidative stress. KW - Nanoparticles KW - Silver KW - Alzheimer's disease KW - Glutathione KW - Antioxidants KW - Serine proteases KW - Brain diseases KW - Metabolomics Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-202696 VL - 14 IS - 11 ER - TY - JOUR A1 - Krauss, Jochen A1 - Vikuk, Veronika A1 - Young, Carolyn A. A1 - Krischke, Markus A1 - Mueller, Martin J. A1 - Baerenfaller, Katja T1 - Epichloë endophyte infection rates and alkaloid content in commercially available grass seed mixtures in Europe JF - Microorganisms N2 - Fungal endophytes of the genus Epichloë live symbiotically in cool season grass species and can produce alkaloids toxic to insects and vertebrates, yet reports of intoxication of grazing animals have been rare in Europe in contrast to overseas. However, due to the beneficial resistance traits observed in Epichloë infected grasses, the inclusion of Epichloë in seed mixtures might become increasingly advantageous. Despite the toxicity of fungal alkaloids, European seed mixtures are rarely tested for Epichloë infection and their infection status is unknown for consumers. In this study, we tested 24 commercially available seed mixtures for their infection rates with Epichloë endophytes and measured the concentrations of the alkaloids ergovaline, lolitrem B, paxilline, and peramine. We detected Epichloë infections in six seed mixtures, and four contained vertebrate and insect toxic alkaloids typical for Epichloë festucae var. lolii infecting Lolium perenne. As Epichloë infected seed mixtures can harm livestock, when infected grasses become dominant in the seeded grasslands, we recommend seed producers to test and communicate Epichloë infection status or avoiding Epichloë infected seed mixtures. KW - Epichloë spp. KW - grass endophytes KW - cool-season grass species KW - infection rates KW - alkaloids KW - toxicity KW - livestock KW - horses KW - Lolium perenne KW - perennial ryegrass Y1 - 2020 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-203323 SN - 2076-2607 VL - 8 IS - 4 ER - TY - JOUR A1 - Elmaidomy, Abeer H. A1 - Mohammed, Rabab A1 - Hassan, Hossam M. A1 - Owis, Asmaa I. A1 - Rateb, Mostafa E. A1 - Khanfar, Mohammad A. A1 - Krischke, Markus A1 - Mueller, Martin J. A1 - Abdelmohsen, Usama Ramadan T1 - Metabolomic profiling and cytotoxic tetrahydrofurofuran lignans investigations from Premna odorata Blanco JF - Metabolites N2 - Metabolomic profiling of different Premna odorata Blanco (Lamiaceae) organs, bark, wood, young stems, flowers, and fruits dereplicated 20, 20, 10, 20, and 20 compounds, respectively, using LC–HRESIMS. The identified metabolites (1–34) belonged to different chemical classes, including iridoids, flavones, phenyl ethanoids, and lignans. A phytochemical investigation of P. odorata bark afforded one new tetrahydrofurofuran lignan, 4β-hydroxyasarinin 35, along with fourteen known compounds. The structure of the new compound was confirmed using extensive 1D and 2D NMR, and HRESIMS analyses. A cytotoxic investigation of compounds 35–38 against the HL-60, HT-29, and MCF-7 cancer cell lines, using the MTT assay showed that compound 35 had cytotoxic effects against HL-60 and MCF-7 with IC50 values of 2.7 and 4.2 µg/mL, respectively. A pharmacophore map of compounds 35 showed two hydrogen bond acceptor (HBA) aligning the phenoxy oxygen atoms of benzodioxole moieties, two aromatic ring features vectored on the two phenyl rings, one hydrogen bond donor (HBD) feature aligning the central hydroxyl group and thirteen exclusion spheres which limit the boundaries of sterically inaccessible regions of the target’s active site. KW - Premna KW - lignan KW - metabolomic KW - cytotoxic KW - pharmacophore map Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-193187 SN - 2218-1989 VL - 9 IS - 10 ER - TY - JOUR A1 - Krauss, Jochen A1 - Vikuk, Veronika A1 - Young, Carolyn A. A1 - Krischke, Markus A1 - Mueller, Martin J. A1 - Baerenfaller, Katja T1 - Correction: Krauss, J., et al. Epichloë endophyte infection rates and alkaloid content in commercially available grass seed mixtures in Europe. Microorganisms 2020, 8, 498 JF - Microorganisms N2 - No abstract available. KW - Epichloë KW - endophyte Y1 - 2020 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-216254 SN - 2076-2607 VL - 8 IS - 10 ER -