TY  - THES
A1  - Nerlich, Kai
T1  - Untersuchung des genetischen Schadens in peripheren Lymphozyten von Dialysepatienten mittels Mikrokerntest und Comet-Assay
T1  - Analysis of genomic damage in peripheral lymphocytes of dialysis patients using the micronucleus- and comet-assay
N2  - Die vorliegende Arbeit beschäftigt sich im Gegensatz zu bisherigen Studien (überwiegend im Rahmen von Querschnittsstudien) v.a. prospektiv mit der Untersuchung der Auswirkung verschiedener Faktoren (1. Prädialysephase, 2. Wechsel von der Hämodialyse zu HDF-Behandlung, 3. Einfluß einer Angiotensin IIAntagonistentherapie sowie (durch einmalige Erhebung) „Langzeit“-HDF-Behandlung)auf den relativen genetischen Schaden, ermittelt durch den Comet-Assay und den Mikrokern-Test in peripheren Lymphozyten bei Dialysepatienten bzw. Prädialysepatienten.
N2  - In end-stage renal failure cancer incidence is enhanced. We are analysing the DNA damage in peripheral lymphocytes of (pre-)dialysis patients using two biomarkers: micronuclei frequency and single cell gel electrophoresis (comet assay)to evaluate the most beneficial therapy.
KW  - genetischer Schadens
KW  - periphere Lymphozyten
KW  - Dialysepatienten
KW  - Mikrokerntest
KW  - Comet-Assay
KW  - genomic damage
KW  - peripheral lymphocytes
KW  - dialysis patients
KW  - micronucleus- assay
KW  - comet-assay
Y1  - 2004
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-9726
ER  - 
TY  - JOUR
A1  - Aster, Hans-Christoph
A1  - Romanos, Marcel
A1  - Walitza, Susanne
A1  - Gerlach, Manfred
A1  - Mühlberger, Andreas
A1  - Rizzo, Albert
A1  - Andreatta, Marta
A1  - Hasenauer, Natalie
A1  - Hartrampf, Philipp E.
A1  - Nerlich, Kai
A1  - Reiners, Christoph
A1  - Lorenz, Reinhard
A1  - Buck, Andreas K.
A1  - Deserno, Lorenz
T1  - Responsivity of the striatal dopamine system to methylphenidate — A within-subject I-123-β-CIT-SPECT study in male children and adolescents with attention-deficit/hyperactivity disorder
JF  - Frontiers in Psychiatry
N2  - Background:
Methylphenidate (MPH) is the first-line pharmacological treatment of attention-deficit/hyperactivity disorder (ADHD). MPH binds to the dopamine (DA) transporter (DAT), which has high density in the striatum. Assessments of the striatal dopamine transporter by single positron emission computed tomography (SPECT) in childhood and adolescent patients are rare but can provide insight on how the effects of MPH affect DAT availability. The aim of our within-subject study was to investigate the effect of MPH on DAT availability and how responsivity to MPH in DAT availability is linked to clinical symptoms and cognitive functioning.


Methods
Thirteen adolescent male patients (9–16 years) with a diagnosis of ADHD according to the DSM-IV and long-term stimulant medication (for at least 6 months) with MPH were assessed twice within 7 days using SPECT after application of I-123-β-CIT to examine DAT binding potential (DAT BP). SPECT measures took place in an on- and off-MPH status balanced for order across participants. A virtual reality continuous performance test was performed at each time point. Further clinical symptoms were assessed for baseline off-MPH.


Results
On-MPH status was associated with a highly significant change (−29.9%) of striatal DAT BP as compared to off-MPH (t = −4.12, p = 0.002). A more pronounced change in striatal DAT BP was associated with higher off-MPH attentional and externalizing symptom ratings (Pearson r = 0.68, p = 0.01). Striatal DAT BP off-MPH, but not on-MPH, was associated with higher symptom ratings (Pearson r = 0.56, p = 0.04).


Conclusion
Our findings corroborate previous reports from mainly adult samples that MPH changes striatal DAT BP availability and suggest higher off-MPH DAT BP, likely reflecting low baseline DA levels, as a marker of symptom severity.
KW  - methylphenidate
KW  - attention deficit/hyperactivity disorder (ADHD)
KW  - striatum
KW  - single photon emission computed tomography (SPECT)
KW  - responsivity
KW  - caudate nucleus
KW  - dopamine transporter (DAT)
Y1  - 2022
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-270862
SN  - 1664-0640
VL  - 13
ER  -