TY  - JOUR
A1  - Hazur, Jonas
A1  - Detsch, Rainer
A1  - Karakaya, Emine
A1  - Kaschta, Joachim
A1  - Teßmar, Jörg
A1  - Schneidereit, Dominik
A1  - Friedrich, Oliver
A1  - Schubert, Dirk W
A1  - Boccaccini, Aldo R
T1  - Improving alginate printability for biofabrication: establishment of a universal and homogeneous pre-crosslinking technique
JF  - Biofabrication
N2  - Many different biofabrication approaches as well as a variety of bioinks have been developed by researchers working in the field of tissue engineering. A main challenge for bioinks often remains the difficulty to achieve shape fidelity after printing. In order to overcome this issue, a homogeneous pre-crosslinking technique, which is universally applicable to all alginate-based materials, was developed. In this study, the Young’s Modulus after post-crosslinking of selected hydrogels, as well as the chemical characterization of alginate in terms of M/G ratio and molecular weight, were determined. With our technique it was possible to markedly enhance the printability of a 2% (w/v) alginate solution, without using a higher polymer content, fillers or support structures. 3D porous scaffolds with a height of around 5 mm were printed. Furthermore, the rheological behavior of different pre-crosslinking degrees was studied. Shear forces on cells as well as the flow profile of the bioink inside the printing nozzle during the process were estimated. A high cell viability of printed NIH/3T3 cells embedded in the novel bioink of more than 85% over a time period of two weeks could be observed.
KW  - alginate
KW  - bioprinting
KW  - rheology
KW  - bioink
KW  - pre-crosslinking
KW  - printability
KW  - shape fidelity
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-254030
VL  - 12
IS  - 4
ER  -