TY  - JOUR
A1  - Hausmann, Stefan
A1  - Brandt, Evelyn
A1  - Köchel, Carolin
A1  - Einsele, Hermann
A1  - Bargou, Ralf C.
A1  - Seggewiss-Bernhardt, Ruth
A1  - Stühmer, Thorsten
T1  - Loss of serum and glucocorticoid-regulated kinase 3 (SGK3) does not affect proliferation and survival of multiple myeloma cell lines
JF  - PLoS ONE
N2  - Multiple myeloma (MM) is a generally fatal plasma cell cancer that often shows activation of the phosphoinositide 3-kinase/Akt (PI3K/Akt) pathway. Targeted pharmacologic therapies, however, have not yet progressed beyond the clinical trial stage, and given the complexity of the PI3K/Akt signalling system (e.g. multiple protein isoforms, diverse feedback regulation mechanisms, strong variability between patients) it is mandatory to characterise its ramifications in order to better guide informed decisions about the best therapeutic approaches. Here we explore whether serum and glucocorticoid-regulated kinase 3 (SGK3), a potential downstream effector of PI3K, plays a role in oncogenic signalling in MM cells-either in concert with or independent of Akt. SGK3 was expressed in all MM cell lines and in all primary MM samples tested. Four MM cell lines representing a broad range of intrinsic Akt activation (very strong: MM. 1s, moderate: L 363 and JJN-3, absent: AMO-1) were chosen to test the effects of transient SGK3 knockdown alone and in combination with pharmacological inhibition of Akt, PI3K-p110\(\alpha\), or in the context of serum starvation. Although the electroporation protocol led to strong SGK3 depletion for at least 5 days its absence had no substantial effect on the activation status of potential downstream substrates, or on the survival, viability or proliferation of MM cells in all experimental contexts tested. We conclude that it is unlikely that SGK3 plays a significant role for oncogenic signalling in multiple myeloma.
KW  - Akt
KW  - phosphorylation
KW  - downstream
KW  - mechanism
KW  - pathway
KW  - isoforms
KW  - activation
KW  - cancer
KW  - inhibition
KW  - phosphatidylinositol 3-kinase/Akt
Y1  - 2015
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-148708
VL  - 10
IS  - 4
ER  - 
TY  - JOUR
A1  - Hardulak, Laura A.
A1  - Morinière, Jérôme
A1  - Hausmann, Axel
A1  - Hendrich, Lars
A1  - Schmidt, Stefan
A1  - Doczkal, Dieter
A1  - Müller, Jörg
A1  - Hebert, Paul D. N.
A1  - Haszprunar, Gerhard
T1  - DNA metabarcoding for biodiversity monitoring in a national park: Screening for invasive and pest species
JF  - Molecular Ecology Resources
N2  - DNA metabarcoding was utilized for a large‐scale, multiyear assessment of biodiversity in Malaise trap collections from the Bavarian Forest National Park (Germany, Bavaria). Principal component analysis of read count‐based biodiversities revealed clustering in concordance with whether collection sites were located inside or outside of the National Park. Jaccard distance matrices of the presences of barcode index numbers (BINs) at collection sites in the two survey years (2016 and 2018) were significantly correlated. Overall similar patterns in the presence of total arthropod BINs, as well as BINs belonging to four major arthropod orders across the study area, were observed in both survey years, and are also comparable with results of a previous study based on DNA barcoding of Sanger‐sequenced specimens. A custom reference sequence library was assembled from publicly available data to screen for pest or invasive arthropods among the specimens or from the preservative ethanol. A single 98.6% match to the invasive bark beetle Ips duplicatus was detected in an ethanol sample. This species has not previously been detected in the National Park.
KW  - biodiversity
KW  - DNA barcoding
KW  - invasive species
KW  - metabarcoding
KW  - monitoring
KW  - pest species
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-217812
VL  - 20
IS  - 6
SP  - 1542
EP  - 1557
ER  -