TY  - JOUR
A1  - Holzapfel, Boris Michael
A1  - Rechl, Hans
A1  - Lehner, Stefan
A1  - Pilge, Hakan
A1  - Gollwitzer, Hans
A1  - Steinhauser, Erwin
T1  - Alloplastic Reconstruction of the Extensor Mechanism after Resection of Tibial Sarcoma [Research Article]
N2  - Reconstruction of the extensor mechanism is essential for good extremity function after endoprosthetic knee replacement following tumor resection. Only a few biological methods have been able to reliably restore a functional extensor mechanism, but they are often associated with significant complication rates. Reattachment of the patellar tendon to the prosthesis using an alloplastic patellar ligament (Trevira cord) can be an appropriate alternative. In vivo and in vitro studies have already shown that complete fibrous ingrowth in polyethylene chords can be seen after a period of six months. However, until now, no biomechanical study has shown the efficacy of an alloplastic cord and its fixation device in providing sufficient stability and endurance in daily life-activity until newly formed scar tissue can take over this function. In a special test bench developed for this study, different loading regimes were applied to simulate loads during everyday life. Failure loads and failuremodes were evaluated. The properties of the cord were compared before and after physiological conditioning. It was shown that rubbing was the mode of failure under dynamic loading. Tensile forces up to 2558N did not result in material failure. Thus, using an artificial cord together with this fixation device, temporary sufficient stable fixation can be expected.
KW  - Medizin
Y1  - 2011
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-69072
ER  - 
TY  - JOUR
A1  - Whisnant, Adam W.
A1  - Jürges, Christopher S.
A1  - Hennig, Thomas
A1  - Wyler, Emanuel
A1  - Prusty, Bhupesh
A1  - Rutkowski, Andrzej J.
A1  - L'hernault, Anne
A1  - Djakovic, Lara
A1  - Göbel, Margarete
A1  - Döring, Kristina
A1  - Menegatti, Jennifer
A1  - Antrobus, Robin
A1  - Matheson, Nicholas J.
A1  - Künzig, Florian W. H.
A1  - Mastrobuoni, Guido
A1  - Bielow, Chris
A1  - Kempa, Stefan
A1  - Liang, Chunguang
A1  - Dandekar, Thomas
A1  - Zimmer, Ralf
A1  - Landthaler, Markus
A1  - Grässer, Friedrich
A1  - Lehner, Paul J.
A1  - Friedel, Caroline C.
A1  - Erhard, Florian
A1  - Dölken, Lars
T1  - Integrative functional genomics decodes herpes simplex virus 1
JF  - Nature Communications
N2  - The predicted 80 open reading frames (ORFs) of herpes simplex virus 1 (HSV-1) have been intensively studied for decades. Here, we unravel the complete viral transcriptome and translatome during lytic infection with base-pair resolution by computational integration of multi-omics data. We identify a total of 201 transcripts and 284 ORFs including all known and 46 novel large ORFs. This includes a so far unknown ORF in the locus deleted in the FDA-approved oncolytic virus Imlygic. Multiple transcript isoforms expressed from individual gene loci explain translation of the vast majority of ORFs as well as N-terminal extensions (NTEs) and truncations. We show that NTEs with non-canonical start codons govern the subcellular protein localization and packaging of key viral regulators and structural proteins. We extend the current nomenclature to include all viral gene products and provide a genome browser that visualizes all the obtained data from whole genome to single-nucleotide resolution. Here, using computational integration of multi-omics data, the authors provide a detailed transcriptome and translatome of herpes simplex virus 1 (HSV-1), including previously unidentified ORFs and N-terminal extensions. The study also provides a HSV-1 genome browser and should be a valuable resource for further research.
KW  - infected-cell protein
KW  - messenger RNA
KW  - binding protein
KW  - type 1
KW  - identification
KW  - ICP27
KW  - translation
KW  - expression
KW  - sequence
KW  - domain
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-229884
VL  - 11
ER  -