TY  - JOUR
A1  - Hassan, Musa A.
A1  - Vasquez, Juan J.
A1  - Guo-Liang, Chew
A1  - Meissner, Markus
A1  - Siegel, T. Nicolai
T1  - Comparative ribosome profiling uncovers a dominant role for translational control in \(Toxoplasma\) \(gondii\)
JF  - BMC Genomics
N2  - Background
The lytic cycle of the protozoan parasite \(Toxoplasma\) \(gondii\), which involves a brief sojourn in the extracellular space, is characterized by defined transcriptional profiles. For an obligate intracellular parasite that is shielded from the cytosolic host immune factors by a parasitophorous vacuole, the brief entry into the extracellular space is likely to exert enormous stress. Due to its role in cellular stress response, we hypothesize that translational control plays an important role in regulating gene expression in \(Toxoplasma\) during the lytic cycle. Unlike transcriptional profiles, insights into genome-wide translational profiles of \(Toxoplasma\) \(gondii\) are lacking.

Methods
We have performed genome-wide ribosome profiling, coupled with high throughput RNA sequencing, in intracellular and extracellular \(Toxoplasma\) \(gondii\) parasites to investigate translational control during the lytic cycle.

Results
Although differences in transcript abundance were mostly mirrored at the translational level, we observed significant differences in the abundance of ribosome footprints between the two parasite stages. Furthermore, our data suggest that mRNA translation in the parasite is potentially regulated by mRNA secondary structure and upstream open reading frames.

Conclusion
We show that most of the \(Toxoplasma\) genes that are dysregulated during the lytic cycle are translationally regulated.
KW  - Biology
KW  - Ribosome profiling
KW  - RNA-sequencing
KW  - Translation efficiency
KW  - Toxoplasma gondii
KW  - Apicomplexan
Y1  - 2017
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-172376
VL  - 18
ER  -