TY  - JOUR
A1  - Seher, Axel
A1  - Nickel, Joachim
A1  - Mueller, Thomas D.
A1  - Kneitz, Susanne
A1  - Gebhardt, Susanne
A1  - Meyer ter Vehn, Tobias
A1  - Schlunck, Guenther
A1  - Sebald, Walter
T1  - Gene expression profiling of connective tissue growth factor (CTGF) stimulated primary human tenon fibroblasts reveals an inflammatory and wound healing response in vitro
JF  - Molecular Vision
N2  - Purpose: 

The biologic relevance of human connective tissue growth factor (hCTGF) for primary human tenon fibroblasts (HTFs) was investigated by RNA expression profiling using affymetrix (TM) oligonucleotide array technology to identify genes that are regulated by hCTGF. 

Methods: 

Recombinant hCTGF was expressed in HEK293T cells and purified by affinity and gel chromatography. Specificity and biologic activity of hCTGF was confirmed by biosensor interaction analysis and proliferation assays. For RNA expression profiling HTFs were stimulated with hCTGF for 48h and analyzed using affymetrix (TM) oligonucleotide array technology. Results were validated by real time RT-PCR. 

Results: 

hCTGF induces various groups of genes responsible for a wound healing and inflammatory response in HTFs. A new subset of CTGF inducible inflammatory genes was discovered (e.g., chemokine [C-X-C motif] ligand 1 [CXCL1], chemokine [C-X-C motif] ligand 6 [CXCL6], interleukin 6 [IL6], and interleukin 8 [IL8]). We also identified genes that can transmit the known biologic functions initiated by CTGF such as proliferation and extracellular matrix remodelling. Of special interest is a group of genes, e.g., osteoglycin (OGN) and osteomodulin (OMD), which are known to play a key role in osteoblast biology. 

Conclusions: 

This study specifies the important role of hCTGF for primary tenon fibroblast function. The RNA expression profile yields new insights into the relevance of hCTGF in influencing biologic processes like wound healing, inflammation, proliferation, and extracellular matrix remodelling in vitro via transcriptional regulation of specific genes. The results suggest that CTGF potentially acts as a modulating factor in inflammatory and wound healing response in fibroblasts of the human eye.
KW  - Bone morphogenetic protein-2
KW  - Smooth-muscle-cells
KW  - Myofibroblast differentiation
KW  - TGF-beta
KW  - CYR61
KW  - Proliferation
KW  - Mechanisms
KW  - Apoptosis
KW  - Receptor
KW  - Cancer
Y1  - 2011
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-140189
VL  - 17
IS  - 08. Okt
ER  - 
TY  - JOUR
A1  - Schroeder, Katharina
A1  - Meyer-ter-Vehn, Tobias
A1  - Fassnacht-Riederle, Heidi
A1  - Guthoff, Rainer
T1  - Course of disease in multifocal choroiditis lacking sufficient immunosuppression: a case report
JF  - Journal of Medical Case Reports
N2  - Background:
Multifocal choroiditis with panuveitis is a rare disease. The educational merit of this case presentation results from the good documentation and the impressive ocular fundus pictures.

Case presentation:
We illustrate the 3-year course of disease in a 22-year-old myopic white woman with multifocal choroiditis with panuveitis and secondary choroidal neovascularization. The activity of the disease was evaluated clinically by optical coherence tomography and fluorescein angiography. Choroidal neovascularization was treated by intravitreal bevacizumab (2.5 mg/0.1 ml). Our patient lacked systemic therapy for the first 11 months because of noncompliance.

Conclusions:
The case is remarkable as the delayed onset of peripheral lesions and the additional existence of high myopia made diagnosis difficult. In addition, it demonstrates that full outbreak of disease with multiple central and peripheral fundus lesions and secondary choroidal neovascularization can develop without systemic treatment.
KW  - multifocal choroiditis
KW  - chorioretinal lesions
KW  - secondary CNV
KW  - bevacizumab
KW  - systemic immunosuppression
KW  - case report
Y1  - 2016
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-171317
VL  - 10
IS  - 298
ER  -