TY  - JOUR
A1  - Schick, Martin Alexander
A1  - Baar, Wolfgang
A1  - Bruno, Raphael Romano
A1  - Wollborn, Jakob
A1  - Held, Christopher
A1  - Schneider, Reinhard
A1  - Flemming, Sven
A1  - Schlegel, Nicolas
A1  - Roewer, Norbert
A1  - Neuhaus, Winfried
A1  - Wunder, Christian
T1  - Balanced hydroxyethylstarch (HES 130/0.4) impairs kidney function in-vivo without inflammation
JF  - PLoS One
N2  - Volume therapy is a standard procedure in daily perioperative care, and there is an ongoing discussion about the benefits of colloid resuscitation with hydroxyethylstarch (HES). In sepsis HES should be avoided due to a higher risk for acute kidney injury (AKI). Results of the usage of HES in patients without sepsis are controversial. Therefore we conducted an animal study to evaluate the impact of 6% HES 130/0.4 on kidney integrity with sepsis or under healthy conditions Sepsis was induced by standardized Colon Ascendens Stent Peritonitis (sCASP). sCASP-group as well as control group (C) remained untreated for 24 h. After 18 h sCASP+HES group (sCASP+VOL) and control+HES (C+VOL) received 50 ml/KG balanced 6% HES (VOL) 130/0.4 over 6h. After 24h kidney function was measured via Inulin- and PAH-Clearance in re-anesthetized rats, and serum urea, creatinine (crea), cystatin C and Neutrophil gelatinase-associated lipocalin (NGAL) as well as histopathology were analysed. In vitro human proximal tubule cells (PTC) were cultured +/- lipopolysaccharid (LPS) and with 0.1–4.0% VOL. Cell viability was measured with XTT-, cell toxicity with LDH-test. sCASP induced severe septic AKI demonstrated divergent results regarding renal function by clearance or creatinine measure focusing on VOL. Soleley HES (C+VOL) deteriorated renal function without sCASP. Histopathology revealed significantly derangements in all HES groups compared to control. In vitro LPS did not worsen the HES induced reduction of cell viability in PTC cells. For the first time, we demonstrated, that application of 50 ml/KG 6% HES 130/0.4 over 6 hours induced AKI without inflammation in vivo. Severity of sCASP induced septic AKI might be no longer susceptible to the way of volume expansion
KW  - colloids
KW  - kidneys
KW  - histopathology
KW  - blood
KW  - creatinine
KW  - sepsis
KW  - urine
KW  - inflammation
Y1  - 2015
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-126068
VL  - 10
IS  - 9
ER  - 
TY  - CHAP
A1  - Held, Winfried
T1  - Künstliche Beleuchtung und Architektur
N2  - No abstract available
KW  - Griechenland <Altertum> / Beleuchtung / Architektur
Y1  - 1990
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-39125
ER  - 
TY  - CHAP
A1  - Held, Winfried
T1  - Heiligtum und Wohnhaus : ein Beitrag zur Topographie des klassischen Milet
N2  - No abstract available
KW  - Klassische Archäologie
Y1  - 1993
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-39133
ER  - 
TY  - CHAP
A1  - Held, Winfried
A1  - Rumscheid, Frank
T1  - Erinnerungen an Mozakis. Eine neugefundene Stockwerkstele aus dem bithynischen Tarsos
N2  - No abstract available
KW  - Klassische Archäologie
Y1  - 1994
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-39148
ER  -