TY  - JOUR
A1  - Fan, Ben
A1  - Li, Lei
A1  - Chao, Yanjie
A1  - Förstner, Konrad
A1  - Vogel, Jörg
A1  - Borriss, Rainer
A1  - Wu, Xiao-Qin
T1  - dRNA-Seq Reveals Genomewide TSSs and Noncoding RNAs of Plant Beneficial Rhizobacterium Bacillus amyloliquefaciens FZB42
JF  - PLoS One
N2  - Bacillus amyloliquefaciens subsp. plantarum FZB42 is a representative of Gram-positive plant-growth-promoting rhizobacteria (PGPR) that inhabit plant root environments. In order to better understand the molecular mechanisms of bacteria-plant symbiosis, we have systematically analyzed the primary transcriptome of strain FZB42 grown under rhizospheremimicking conditions using differential RNA sequencing (dRNA-seq). Our analysis revealed 4,877 transcription start sites for protein-coding genes, identified genes differentially expressed under different growth conditions, and corrected many previously mis-annotated genes. We also identified a large number of riboswitches and cis-encoded antisense RNAs, as well as trans-encoded small noncoding RNAs that may play important roles in the gene regulation of Bacillus. Overall, our analyses provided a landscape of Bacillus primary transcriptome and improved the knowledge of rhizobacteria-host interactions.
KW  - gene expression
KW  - subtilis genome
KW  - enterica serovar thphimurium
KW  - small regulatory RNAs
KW  - binding protein HFQ
KW  - escherichia coli
KW  - messenger RNA
KW  - transcriptional landscape
KW  - mycobacterium tuberculosis
KW  - listeria monocytogenes
Y1  - 2015
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-138369
VL  - 10
IS  - 11
ER  -