TY  - JOUR
A1  - Nazzal, Yousef
A1  - Howari, Fares M.
A1  - Yaslam, Aya
A1  - Iqbal, Jibran
A1  - Maloukh, Lina
A1  - Ambika, Lakshmi Kesari
A1  - Al-Taani, Ahmed A.
A1  - Ali, Ijaz
A1  - Othman, Eman M.
A1  - Jamal, Arshad
A1  - Naseem, Muhammad
T1  - A methodological review of tools that assess dust microbiomes, metatranscriptomes and the particulate chemistry of indoor dust
JF  - Atmosphere
N2  - Indoor house dust is a blend of organic and inorganic materials, upon which diverse microbial communities such as viruses, bacteria and fungi reside. Adequate moisture in the indoor environment helps microbial communities multiply fast. The outdoor air and materials that are brought into the buildings by airflow, sandstorms, animals pets and house occupants endow the indoor dust particles with extra features that impact human health. Assessment of the health effects of indoor dust particles, the type of indoor microbial inoculants and the secreted enzymes by indoor insects as allergens merit detailed investigation. Here, we discuss the applications of next generation sequencing (NGS) technology which is used to assess microbial diversity and abundance of the indoor dust environments. Likewise, the applications of NGS are discussed to monitor the gene expression profiles of indoor human occupants or their surrogate cellular models when exposed to aqueous solution of collected indoor dust samples. We also highlight the detection methods of dust allergens and analytical procedures that quantify the chemical nature of indoor particulate matter with a potential impact on human health. Our review is thus unique in advocating the applications of interdisciplinary approaches that comprehensively assess the health effects due to bad air quality in built environments.
KW  - indoor dust
KW  - allergens
KW  - metagenomics
KW  - particulate matter
KW  - microbiomes
KW  - transcriptomes
KW  - health effects
Y1  - 2022
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-285957
SN  - 2073-4433
VL  - 13
IS  - 8
ER  -