TY - THES A1 - Veith, Marina T1 - Langzeitergebnisse nach Versorgung proximaler Humerusfrakturen mittels intramedullärer Drahtosteosynthese nach Kapandji T1 - Outcome after Kapandji pinning of proximal humerus fractures N2 - In vorliegender Dissertation wurden die Langzeitergebnisse hinsichtlich der Lebensqualität der Patienten, die sich im Zeitraum 2002-2010 eine proximale Humerusfraktur zuzogen und mittels intramedullärer Drahtosteosynthese nach Kapandji im König Ludwig Haus in Würzburg operiert wurden, untersucht. Die Nachuntersuchung erfolgte mittels klinischer, radiologischer und sonografischer Untersuchung sowie durch Verwendung des DASH-Fragebogens, Constant Murley Score und der Visuellen Analogskala. N2 - The outcome of patients which got a proximal humerus fracture betweeen 2002-2010 and had been treated with Kapandji pinning in König Ludwig Haus in Würzburg has been investigated in this dissertation. The follow up examination includes clinical investigation, X-ray and ultrasonics as well as DASH form, Constant Murley Score and the visual analogue scale. KW - proximale Humerusfraktur KW - Kapandjidrahtosteosynthese KW - proximale Humerusfraktur KW - Kapandjidrahtosteosynthese KW - proximal humerus fracture KW - Kapandji pinning Y1 - 2020 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-209906 ER - TY - THES A1 - Webering, Isabella T1 - Langzeitergebnisse und Migrationsanalyse sphärischer Pressfit-Pfannen in Kombination mit einer Pfannendachplastik T1 - Long-term results and migration analysis of spherical pressfit cups with femoral head bone graft N2 - Die Versorgung acetabulärer Defektsituationen stellt eine besondere chirurgische Herausforderung dar. Die bisher vorliegenden Ergebnisse der Pfannendachplastik mit zementierten Pfannen zeigen ein zunehmendes Versagen der Versorgung nach mehr als 7 Jahren. Im Gegensatz dazu liegen für zementfreie Pfannen in Kombination mit einer Pfannendachplastik bisher nur begrenzte Ergebnisse vor. Ziel der vorliegenden Studie war die Analyse der eigenen Ergebnisse und eine röntgenologische Wanderungsmessung mit dem Verfahren der Einbildröntgenanalyse (EBRA). Zwischen 1987 und 1992 wurden in unserer Institution 65 Pfannendachplastiken bei 56 Patienten (w:m = 46:10, mittleres Alter 52,3 ± 11,9 Jahre) in Kombination mit der zementfreien Harris-Galante-Pfanne, Typ I, durchgeführt. Diagnosen waren Dysplasiecoxarthrose (n = 56) und Pfannenlockerung (n = 9). Die Klassifikation nach Hartofilakidis lautete Dysplasie (Typ 1 - n = 8), Subluxation (Typ 2 – n = 41) und hohe Dislokation (Typ 3 – n = 16). Alle Implantate wurden nach prospektiven klinischen und radiologischen Kriterien regelmäßig nachuntersucht. Die Implantatmigration wurde retrospektiv mit dem digitalen Messverfahren der Einbildröntgenanalyse (EBRA) mit einer Messgenauigkeit von 1 mm dokumentiert. Nach 10,2 ± 2,9 Jahren waren 4 Patienten mit insgesamt 5 Prothesen verstorben. Von den überlebenden Patienten wurden 5 Implantate gewechselt. Bei 2 weiteren Implantaten bestand ein Lockerungsverdacht. Die Survivorship-Analyse mit dem Kriterium der Revision bzw. der Lockerung ergab eine 11-Jahres-Überlebensrate von 90,4 bzw. 88%. Die Migrationsanalyse zeigte, dass alle gelockerten Implantate im Mittel 2,2 ± 1,8 mm horizontal und 1,1 ± 1,5 mm nach cranial gewandert waren. Von den 58 nicht gelockerten Implantaten zeigten 17 weitere Pfannen Implantatmigrationen von 2,1 ± 0,9 mm in horizontaler Richtung und 0,6 ± 0,6 mm in cranialer Richtung. 38 nicht gelockerte Implantate zeigten eine mittlere Migration von 0,4 ± 0,3 mm horizontal und 0,1 ± 0,2 mm cranial. 3 Implantate ohne Lockerungszeichen konnten nicht vermessen werden. Wurde eine Überlebenszeitanalyse für die nicht gewanderten bzw. gewanderten Implantate durchgeführt, so zeigte sich ein 100%iges Überleben der nicht gewanderten Implantate über 12 Jahre, wohingegen die gewanderten Pfannen eine Überlebensrate von 70% aufwiesen (p=0,001). Korrelationen der Implantatmigration zu dem Alter bei Operation, zum praeoperativen CE-Winkel, der Einteilung nach Hartofilakidis bzw. Crowe, der prozentualen Überdachung durch das Transplantat und der Pfanneninklination zeigten keine signifikanten Zusammenhänge. Dagegen bestehen für die Verwendung eines homologen Transplantates (p = 0,014) und die Einteilung des Rotationszentrums nach Ranawat bzw. Pagnano (p = 0,028) signifikante Korrelationen zur Implantatmigration. Die vorliegende Arbeit beschreibt günstige 10-Jahresergebnisse sphärischer Pressfitpfannen in Kombination mit einer Pfannendachplastik. Es besteht ein signifikanter Zusammenhang zwischen Implantatmigration und Überlebensrate. Die im Vergleich zur Implantation ohne Pfannendachplastik deutlich höhere Migrationsrate lässt allerdings in den nächsten Jahren eine Zunahme der Revisionen in diesem Kollektiv erwarten. N2 - Background: Results which have been obtained up until now for acetabuloplasty with cemented cups have indicated increasing failure after periods of more than 7 years. In contrast to this, only limited results are available for cement-free cups in combination with an acetabuloplasty. The aim of the study presented here was an analysis of our own results and a radiographic migration analysis using the method of single-image x-ray analysis (Einbildroentgenanalyse - EBRA). Methods: Between 1987 and 1992, 65 acetabuloplasties were performed in our institution on 56 patients (f/m = 45:10, average age 52.3 ± 11.9 Jahre) in combination with the cement-free, Type I Harris Galante cup. The diagnoses were dysplasia coxarthrosis (n = 56) and cup loosening (n = 9). The classification according to Hartofilakidis was dysplasia (Type 1 - n = 8), low dislocation (Type 2 – n = 41) and high dislocation (Type 3 – n = 16). All implants were prospectively followed-up clinically and radiographically on a regular basis. In addition, the implant migration was documented retrospectively with the digital measurement method of single-image x-ray analysis with a measurement precision of 1 mm. Results: After 10.2 ± 2.9 years, 4 patients had died. In the group of surviving patients, 5 implants were revised . In two additional cases, there was radiographic evidence of possible acetabular loosening because of progressive radiolucencies. The survivorship analysis with the criterion of revision or loosening produced a 11-year survival rate of 90 or 88 %, respectively. Migration analysis showed that all loosened implants were migrated, on average, 2.2 ± 1.8 mm horizontally and 1.1 ± 1.5 mm in the cranial direction. Of the 58 non-loosened implants, 17 showed cup implant migration of 2.1 ± 0.9 mm in the horizontal direction and 0.6 ± 0.6 mm in the cranial direction. 38 non-loosened implants showed an average migration of 0.4 ± 0.3 mm horizontally and 0.1 ± 0.2 mm in the cranial direction. 3 implants without any indications of loosening could not be measured. When a survival period analysis for the non-migrated or migrated implants was accomplished, there was a 100 % survival of the non-migrated implants over 12 years, whereas the migrated cups showed a survival rate of 70 % (p = 0.001). Correlation analysis of implant migration with age at the time of the operation, with the pre-operative CE angle, the classification according to Hartofilakidis or Crowe, respectively, the percentage covering by the transplant and the cup inclination revealed no significant correlations. In contrast, the use of an allograft implant (p = 0.014) and the location of the rotation centre according to Ranawat or Pagnano, respectively, were significantly correlated with implant migration (p = 0.028). Conclusions: The 90% eleven year survival rate for spherical press-fit cups in combination with an acetabuloplasty is satisfactory, given the complexity of these reconstructions. There is a significant correlation between implant migration and a decreased survival rate. The higher rate of migration compared with implantation without acetabuloplasty allows one, however, to expect an increasing revision in this collective in the coming years. KW - Migrationsanalyse KW - EBRA KW - Pfannendachplastik KW - sphärische Pressfit-Pfanne KW - migration analysis KW - EBRA KW - femoral head bone graft KW - spherical pressfit cup Y1 - 2002 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-5409 ER - TY - THES A1 - Knödler, Eva T1 - Langzeitergebnisse über 12-16 Jahre für die Hybrid-Knieendoprothese PFC-Modular T1 - Longterm results over 12-16 years for Hybrid-TKA PFC-Modular N2 - Ziel der vorliegenden Studie war, die Überlebensrate der Hybrid-PFC-Knieprothese im Langzeit-verlauf darzustellen. Insgesamt wurden 91 Patienten erfasst (50 mit klinischer und radiologischer Untersuchung und 41 anhand der Fragebögen). Es handelt sich um 78 Frauen und 13 Männer mit einem durchschnittlichen Alter von 79,6 Jahren zum Nachuntersuchungszeitpunkt (März bis Juni 2004). Der Nachuntersuchungszeitraum umfasst 12 - 16 Jahre nach Implantation. Ausgewertet wurden die folgenden standartisierten Fragebögen: WOMAC, SMFA-D, KSS. Die radiologische Untersuchung erfolgte gemäß KSRESS. Die Überlebenszeitanalyse beträgt 88% nach 15 Jahren. In diesem Zeitraum wurden 10 Revisionen erfasst. WOMAC mit insgesamt guten Ergebnissen bezüglich Schmerz (3,2), Steife (3,6), Funktion (4,1) und Gesamtscore (3,7). SMFA-D mit eher mittelmäßigen Werten von 42,8 im Funktionsindex und 42,4 im Beeinträchtigungsindex und der Knee Society Score (KSS) mit einem mäßigen Ergebnis von 78,8 im Kniescore und einem schlechten Wert 53,6 im Funktionsscore. Die radiologische Auswertung gemäß KSRESS zeigte Aufhellungslinien > 2mm im Bereich D_16_1 1 (Femurschild Position 1) und größere Osteolysen (1 x 8mm und 1 x 10mm) im Bereich D16_4_2 (dorsales Tibiaplateau seitlich), jedoch keine Komponentenlockerung. Die vorliegende Studie zeigt mittelgradige Ergebnisse in den erhobenen Scores bei höherem Durchschnittsalter der erhobenen Studienteilnehmer gegenüber Vergleichs-studien. Die Langzeit-Überlebensrate von 88% zeigt sich ebenbürtig gegenüber anderen Verfahren. Die kürzere OP-Zeit beim Hybridverfahren ist als Vorteil zu sehen. Somit bietet die Hybridtechnik eine gute zusätzliche chirurgische Option in der Knieendoprothetik. Zusammenfassend kann hier berichtet werden das die PFC-Modular Hybrid-Knieendo-prothese insgesamt eine gute Haltbarkeit über einen Zeitraum bis zu 16 Jahre bietet. Die eher mäßigen Scores der Fragebögen wurden vermutlich durch das hohe Alter der Patienten beeinflußt. N2 - This thesis shows the results of the survivorship analysis of PFC-Modular TKA in hybrid-technique over 12-16 years. KW - Knieendoprothese KW - Langzeitstudie Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-191019 ER - TY - JOUR A1 - Reichel, Thomas A1 - Herz, Stefan A1 - el Tabbakh, Mohammed A1 - Bley, Thorsten Alexander A1 - Plumhoff, Piet A1 - Rueckl, Kilian T1 - Less than 9.5-mm coracohumeral distance on axial magnetic resonance imaging scans predicts for subscapularis tear JF - JSES International N2 - Background Diagnosis of subscapularis (SSC) tendon lesions on magnetic resonance imaging (MRI) can be challenging. A small coracohumeral distance (CHD) has been associated with SSC tears. This study was designed to define a specific threshold value for CHD to predict SSC tears on axial MRI scans. Methods This retrospective study included 172 shoulders of 168 patients who underwent arthroscopic surgery for rotator cuff tear or glenohumeral instability. Diagnostic arthroscopy confirmed an SSC tear in 62 cases (36.0%, test group a), rotator cuff tear tears other than SSC in 71 cases (41.3%, control group b) and glenohumeral instability without any rotator cuff tear in 39 cases (22.7%, zero-sample group c). All patients had a preoperative MRI of the shoulder (1.5T or 3T). Minimum CHD was measured on axial fat-suppressed proton density-, T2-, or T1-weigthed sequences. Receiver operating characteristics analysis was used to determine the threshold value for CHD, and sensitivity and specificity were calculated. Results CHD measurement had a good interobserver reliability (Intraclass correlation coefficient 0.799). Mean CHD was highly significantly (P < .001) less for test group a (mean 7.3 mm, standard deviation ± 2.2) compared with control group b (mean 11.1 mm, standard deviation ± 2.3) or zero-sample group c (mean 13.6 mm, standard deviation ± 2.9). A threshold value of CHD <9.5 mm had a sensitivity of 83.6% and a specificity of 83.9% to predict SSC tears. Conclusion A CHD <9.5 mm on MRI is predictive of SSC lesions and a valuable tool to diagnose SSC tears. KW - subscapularis tear KW - coracohumeral distance KW - rotator cuff tear KW - coracohumeral impingement Y1 - 2021 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-259118 VL - 5 IS - 3 ER - TY - JOUR A1 - Scorcelletti, Matteo A1 - Kara, Serhan A1 - Zange, Jochen A1 - Jordan, Jens A1 - Semler, Oliver A1 - Schönau, Eckhard A1 - Rittweger, Jörn A1 - Ireland, Alex A1 - Seefried, Lothar T1 - Lower limb bone geometry in adult individuals with X-linked hypophosphatemia: an observational study JF - Osteoporosis International N2 - Summary We assessed lower-limb geometry in adults with X-linked hypophosphatemia (XLH) and controls. We found large differences in multiple measures including femoral and tibial torsion, bowing and cross-sectional area and acetabular version and coverage which may contribute to clinical problems such as osteoarthritis, fractures and altered gait common in XLH. Purpose Individuals with X-linked hypophosphatemia (XLH) are at risk of lower-limb deformities and early onset of osteoarthritis. These two factors may be linked, as altered biomechanics is a risk factor for osteoarthritis. This exploratory evaluation aims at providing clues and concepts for this association to facilitate future larger-scale and longitudinal studies on that aspect. Methods For this observational study, 13 patients with XLH, aged 18–65 years (6 female), were compared with sex-, age- and weight-matched healthy individuals at a single German research centre. Femoral and hip joint geometry, including femoral and tibial torsion and femoral and tibial shaft bowing, bone cross-sectional area (CSA) and acetabular version and coverage were measured from magnetic resonance imaging (MRI) scans. Results Total femoral torsion was 29° lower in individuals with XLH than in controls (p < 0.001), mainly resulting from lower intertrochanteric torsion (ITT) (p < 0.001). Femoral lateral and frontal bowing, tibial frontal bowing, mechanical axis, femoral mechanical–anatomical angle, acetabular version and acetabular coverage were all greater and tibial torsion lower in individuals with XLH as compared to controls (all p < 0.05). Greater femoral total and marrow cavity CSA, greater tibial marrow cavity CSA and lower cortical CSA were observed in XLH (all p < 0.05). Discussion We observed large differences in clinically relevant measures of tibia and particularly femur bone geometry in individuals with XLH compared to controls. These differences may plausibly contribute to clinical manifestations of XLH such as early-onset osteoarthritis, pseudofractures and altered gait and therefore should be considered when planning corrective surgeries. KW - bone KW - femur KW - geometry KW - shape KW - XLH Y1 - 2022 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-324655 VL - 33 IS - 7 ER - TY - JOUR A1 - Chaudry, Oliver A1 - Grimm, Alexandra A1 - Friedberger, Andreas A1 - Kemmler, Wolfgang A1 - Uder, Michael A1 - Jakob, Franz A1 - Quick, Harald H. A1 - von Stengel, Simon A1 - Engelke, Klaus T1 - Magnetic Resonance Imaging and Bioelectrical Impedance Analysis to Assess Visceral and Abdominal Adipose Tissue JF - Obesity N2 - Objective This study aimed to compare a state‐of‐the‐art bioelectrical impedance analysis (BIA) device with two‐point Dixon magnetic resonance imaging (MRI) for the quantification of visceral adipose tissue (VAT) as a health‐related risk factor. Methods A total of 63 male participants were measured using a 3‐T MRI scanner and a segmental, multifrequency BIA device. MRI generated fat fraction (FF) maps, in which VAT volume, total abdominal adipose tissue volume, and FF of visceral and total abdominal compartments were quantified. BIA estimated body fat mass and VAT area. Results Coefficients of determination between abdominal (r\(^{2}\) = 0.75) and visceral compartments (r\(^{2}\) = 0.78) were similar for both groups, but slopes differed by a factor of two. The ratio of visceral to total abdominal FF was increased in older men compared with younger men. This difference was not detected with BIA. MRI and BIA measurements of the total abdominal volume correlated moderately (r\(^{2}\) = 0.31‐0.56), and visceral measurements correlated poorly (r\(^{2}\) = 0.13‐0.44). Conclusions Visceral BIA measurements agreed better with MRI measurements of the total abdomen than of the visceral compartment, indicating that BIA visceral fat area assessment cannot differentiate adipose tissue between visceral and abdominal compartments in young and older participants. Y1 - 2020 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-213591 VL - 28 IS - 2 SP - 277 EP - 283 ER - TY - JOUR A1 - Nedopil, Alexander J. A1 - Howell, Stephen M. A1 - Hull, Maury L. T1 - Measurement of tibial orientation helps select the optimal insert thickness to personalize PCL tension in a medial ball-in-socket TKA JF - Journal of Personalized Medicine N2 - As the conformity of a medial ball-in-socket total knee arthroplasty (TKA) provides intrinsic anterior-posterior (A-P) stability, surgeons cannot rely on the manual examination of sagittal laxity to identify the optimal insert thickness. Instead, the present study determined whether measuring tibial axial orientation in extension and 90° flexion with an insert goniometer could identify the optimal thickness that, when implanted, provides high postoperative function. In twenty-two patients that underwent unrestricted caliper-verified kinematic alignment (KA) with a PCL retaining implant, two surgeons measured tibial orientation in extension and 90° flexion with 10, 11, 12, and 13 mm thick insert goniometers. Each TKA had one insert thickness that restored either the maximum external tibial orientation in extension, the maximum internal tibial orientation at 90° flexion, or both relative to 1 mm thinner and thicker inserts. In addition, the 6-month median [interquartile range] Forgotten Joint Score of 73 (54–87) and Oxford Knee Score of 42 (38–45) indicated high satisfaction and function. In conclusion, surgeons using a medial ball-in-socket TKA design can measure external tibial orientation in extension and internal tibial orientation at 90° flexion with an insert goniometer. Furthermore, implanting an insert with the thickness that provided the maximum orientation values resulted in high postoperative function, thereby personalizing PCL tension. KW - posterior cruciate ligament KW - tibial rotation KW - medial pivot KW - total knee arthroplasty KW - kinematic alignment Y1 - 2022 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-286232 SN - 2075-4426 VL - 12 IS - 9 ER - TY - JOUR A1 - Grote, Stefan A1 - Noeldeke, Tatjana A1 - Blauth, Michael A1 - Mutschler, Wolf A1 - Bürklein, Dominik T1 - Mechanical torque measurement in the proximal femur correlates to failure load and bone mineral density ex vivo JF - Orthopedic Reviews (Pavia) N2 - Knowledge of local bone quality is essential for surgeons to determine operation techniques. A device for intraoperative measurement of local bone quality has been developed by the AO-Research Foundation (DensiProbe®). We used this device to experimentally measure peak breakaway torque of trabecular bone in the proximal femur and correlated this with local bone mineral density (BMD) and failure load. Bone mineral density of 160 cadaver femurs was measured by ex situ dual-energy X-ray absorptiometry. The failure load of all femurs was analyzed by side-impact analysis. Femur fractures were fixed and mechanical peak torque was measured with the DensiProbe® device. Correlation was calculated whereas correlation coefficient and significance was calculated by Fisher’s Z-transformation. Moreover, linear regression analysis was carried out. The unpaired Student’s t-test was used to assess the significance of differences. The Ward triangle region had the lowest BMD with 0.511 g/cm2 (±0.17 g/cm2), followed by the upper neck region with 0.546 g/cm2 (±0.16 g/cm2), trochanteric region with 0.685 g/cm2 (±0.19 g/cm2) and the femoral neck with 0.813 g/cm2 (±0.2 g/cm2). Peak torque of DensiProbe® in the femoral head was 3.48 Nm (±2.34 Nm). Load to failure was 4050.2 N (±1586.7 N). The highest correlation of peak torque measured by Densi Probe® and load to failure was found in the femoral neck (r=0.64, P<0.001). The overall correlation of mechanical peak torque with T-score was r=0.60 (P<0.001). A correlation was found between mechanical peak torque, load to failure of bone and BMD in vitro. Trabecular strength of bone and bone mineral density are different aspects of bone strength, but a correlation was found between them. Mechanical peak torque as measured may contribute additional information about bone strength, especially in the perioperative testing. KW - hip fracture KW - bone mineral density KW - osteoporosis KW - mechanical torque measurement KW - failure load Y1 - 2013 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-132358 VL - 5 IS - e16 ER - TY - JOUR A1 - Ebert, Regina A1 - Dotterweich, Julia A1 - Kraus, Sabrina A1 - Tower, Robert J. A1 - Jakob, Franz A1 - Schütze, Norbert T1 - Mesenchymal stem cell contact promotes CCN1 splicing and transcription in myeloma cells N2 - CCN family member 1 (CCN1), also known as cysteine-rich angiogenic inducer 61 (CYR61), belongs to the extracellular matrix-associated CCN protein family. The diverse functions of these proteins include regulation of cell migration, adhesion, proliferation, differentiation and survival/apoptosis, induction of angiogenesis and cellular senescence. Their functions are partly overlapping, largely non-redundant, cell-type specific, and depend on the local microenvironment. To elucidate the role of CCN1 in the crosstalk between stromal cells and myeloma cells, we performed co-culture experiments with primary mesenchymal stem cells (MSC) and the interleukin-6 (IL-6)-dependent myeloma cell line INA-6. Here we show that INA-6 cells display increased transcription and induction of splicing of intron-retaining CCN1 pre-mRNA when cultured in contact with MSC. Protein analyses confirmed that INA-6 cells co-cultured with MSC show increased levels of CCN1 protein consistent with the existence of a pre-mature stop codon in intron 1 that abolishes translation of unspliced mRNA. Addition of recombinant CCN1-Fc protein to INA-6 cells was also found to induce splicing of CCN1 pre-mRNA in a concentration-dependent manner. Only full length CCN1-Fc was able to induce mRNA splicing of all introns, whereas truncated recombinant isoforms lacking domain 4 failed to induce intron splicing. Blocking RGD-dependent integrins on INA-6 cells resulted in an inhibition of these splicing events. These findings expand knowledge on splicing of the proangiogenic, matricellular factor CCN1 in the tumor microenvironment. We propose that contact with MSC-derived CCN1 leads to splicing and enhanced transcription of CCN1 which further contributes to the translation of angiogenic factor CCN1 in myeloma cells, supporting tumor viability and myeloma bone disease. KW - CCN1 KW - Multiple myeloma KW - Mesenchymal stem cells KW - Splicing Y1 - 2014 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-110497 ER - TY - JOUR A1 - Wagenbrenner, Mike A1 - Poker, Konrad A1 - Heinz, Tizian A1 - Herrmann, Marietta A1 - Horas, Konstantin A1 - Ebert, Regina A1 - Mayer-Wagner, Susanne A1 - Holzapfel, Boris M. A1 - Rudert, Maximilian A1 - Steinert, Andre F. A1 - Weißenberger, Manuel T1 - Mesenchymal stromal cells (MSCs) isolated from various tissues of the human arthritic knee joint possess similar multipotent differentiation potential JF - Applied Sciences N2 - (1) Background: The mesenchymal stromal cells (MSCs) of different tissue origins are applied in cell-based chondrogenic regeneration. However, there is a lack of comparability determining the most suitable cell source for the tissue engineering (TE) of cartilage. The purpose of this study was to compare the in vitro chondrogenic potential of MSC-like cells from different tissue sources (bone marrow, meniscus, anterior cruciate ligament, synovial membrane, and the infrapatellar fat pad removed during total knee arthroplasty (TKA)) and define which cell source is best suited for cartilage regeneration. (2) Methods: MSC-like cells were isolated from five donors and expanded using adherent monolayer cultures. Differentiation was induced by culture media containing specific growth factors. Transforming growth factor (TGF)-ß1 was used as the growth factor for chondrogenic differentiation. Osteogenesis and adipogenesis were induced in monolayer cultures for 27 days, while pellet cell cultures were used for chondrogenesis for 21 days. Control cultures were maintained under the same conditions. After, the differentiation period samples were analyzed, using histological and immunohistochemical staining, as well as molecularbiological analysis by RT-PCR, to assess the expression of specific marker genes. (3) Results: Plastic-adherent growth and in vitro trilineage differentiation capacity of all isolated cells were proven. Flow cytometry revealed the clear co-expression of surface markers CD44, CD73, CD90, and CD105 on all isolated cells. Adipogenesis was validated through the formation of lipid droplets, while osteogenesis was proven by the formation of calcium deposits within differentiated cell cultures. The formation of proteoglycans was observed during chondrogenesis in pellet cultures, with immunohistochemical staining revealing an increased relative gene expression of collagen type II. RT-PCR proved an elevated expression of specific marker genes after successful differentiation, with no significant differences regarding different cell source of native tissue. (4) Conclusions: Irrespective of the cell source of native tissue, all MSC-like cells showed multipotent differentiation potential in vitro. The multipotent differentiation capacity did not differ significantly, and chondrogenic differentiation was proven in all pellet cultures. Therefore, cell suitability for cell-based cartilage therapies and tissue engineering is given for various tissue origins that are routinely removed during total knee arthroplasty (TKA). This study might provide essential information for the clinical tool of cell harvesting, leading to more flexibility in cell availability. KW - knee joint KW - MSCs KW - cellular origin KW - cartilage regeneration KW - tissue engineering KW - cell-based therapies KW - osteoarthritis Y1 - 2022 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-262334 SN - 2076-3417 VL - 12 IS - 4 ER -