TY  - JOUR
A1  - Bangalore, Disha M.
A1  - Heil, Hannah S.
A1  - Mehringer, Christian F.
A1  - Hirsch, Lisa
A1  - Hemmen, Katharina
A1  - Heinze, Katrin G.
A1  - Tessmer, Ingrid
T1  - Automated AFM analysis of DNA bending reveals initial lesion sensing strategies of DNA glycosylases
JF  - Scientific Reports
N2  - Base excision repair is the dominant DNA repair pathway of chemical modifications such as deamination, oxidation, or alkylation of DNA bases, which endanger genome integrity due to their high mutagenic potential. Detection and excision of these base lesions is achieved by DNA glycosylases. To investigate the remarkably high efficiency in target site search and recognition by these enzymes, we applied single molecule atomic force microscopy (AFM) imaging to a range of glycosylases with structurally different target lesions. Using a novel, automated, unbiased, high-throughput analysis approach, we were able to resolve subtly different conformational states of these glycosylases during DNA lesion search. Our results lend support to a model of enhanced lesion search efficiency through initial lesion detection based on altered mechanical properties at lesions. Furthermore, its enhanced sensitivity and easy applicability also to other systems recommend our novel analysis tool for investigations of diverse, fundamental biological interactions.
KW  - atomic-force microscopy
KW  - base pairs
KW  - molecular structure
KW  - crystal structure
KW  - structural basis
KW  - repair
KW  - recognition
KW  - 8-oxoguanine
KW  - thymine
KW  - mismatches
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:bvb:20-opus-231338
VL  - 10
ER  -