The Candidate Phylum Poribacteria by Single-Cell Genomics: New Insights into Phylogeny, Cell-Compartmentation, Eukaryote-Like Repeat Proteins, and Other Genomic Features

Please always quote using this URN: urn:nbn:de:bvb:20-opus-112649
  • The candidate phylum Poribacteria is one of the most dominant and widespread members of the microbial communities residing within marine sponges. Cell compartmentalization had been postulated along with their discovery about a decade ago and their phylogenetic association to the Planctomycetes, Verrucomicrobia, Chlamydiae superphylum was proposed soon thereafter. In the present study we revised these features based on genomic data obtained from six poribacterial single cells. We propose that Poribacteria form a distinct monophyletic phylumThe candidate phylum Poribacteria is one of the most dominant and widespread members of the microbial communities residing within marine sponges. Cell compartmentalization had been postulated along with their discovery about a decade ago and their phylogenetic association to the Planctomycetes, Verrucomicrobia, Chlamydiae superphylum was proposed soon thereafter. In the present study we revised these features based on genomic data obtained from six poribacterial single cells. We propose that Poribacteria form a distinct monophyletic phylum contiguous to the PVC superphylum together with other candidate phyla. Our genomic analyses supported the possibility of cell compartmentalization in form of bacterial microcompartments. Further analyses of eukaryote-like protein domains stressed the importance of such proteins with features including tetratricopeptide repeats, leucin rich repeats as well as low density lipoproteins receptor repeats, the latter of which are reported here for the first time from a sponge symbiont. Finally, examining the most abundant protein domain family on poribacterial genomes revealed diverse phyH family proteins, some of which may be related to dissolved organic posphorus uptake.show moreshow less

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Metadaten
Author: Ute Hentschel, Janine Kamke, Christian Rinke, Patrick Schwientek, Kostas Mavromatis Mavromatis, Natalia Ivanova, Alexander Sczyrba, Tanja Woyke
URN:urn:nbn:de:bvb:20-opus-112649
Document Type:Journal article
Faculties:Fakultät für Biologie / Julius-von-Sachs-Institut für Biowissenschaften
Language:English
Year of Completion:2014
Source:PLoS ONE 9(1): e87353. doi:10.1371/journal.pone.0087353
DOI:https://doi.org/10.1371/journal.pone.0087353
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
Tag:Candidate Phylum Poribacteria; genome analysis; genomic databases; marine bacteria; phylogenetic analysis; phylogenetics; polyvinyl chloride; protein domains; sponges
Release Date:2015/05/07
Note:
This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
Collections:Open-Access-Publikationsfonds / Förderzeitraum 2014
Licence (German):License LogoCC 0: Public Domain Dedication