Characterization of Metastasis Formation and Virotherapy in the Human C33A Cervical Cancer Model

Please always quote using this URN: urn:nbn:de:bvb:20-opus-119674
  • More than 90% of cancer mortalities are due to cancer that has metastasized. Therefore, it is crucial to intensify research on metastasis formation and therapy. Here, we describe for the first time the metastasizing ability of the human cervical cancer cell line C33A in athymic nude mice after subcutaneous implantation of tumor cells. In this model, we demonstrated a steady progression of lumbar and renal lymph node metastases during tumor development. Besides predominantly occurring lymphatic metastases, we visualized the formation ofMore than 90% of cancer mortalities are due to cancer that has metastasized. Therefore, it is crucial to intensify research on metastasis formation and therapy. Here, we describe for the first time the metastasizing ability of the human cervical cancer cell line C33A in athymic nude mice after subcutaneous implantation of tumor cells. In this model, we demonstrated a steady progression of lumbar and renal lymph node metastases during tumor development. Besides predominantly occurring lymphatic metastases, we visualized the formation of hematogenous metastases utilizing red fluorescent protein (RFP) expressing C33A-RFP cells. RFP positive cancer cells were found migrating in blood vessels and forming micrometastases in lungs of tumor-bearing mice. Next, we set out to analyze the influence of oncolytic virotherapy in the C33A-RFP model and demonstrated an efficient virus-mediated reduction of tumor size and metastatic burden. These results suggest the C33A-RFP cervical cancer model as a new platform to analyze cancer metastases as well as to test novel treatment options to combat metastases.show moreshow less

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Metadaten
Author: Ulrike Donat, Juliane Rother, Simon Schäfer, Michael Hess, Barbara Härtl, Christina Kober, Johanna Langbein-Laugwitz, Jochen Stritzker, Nanhai G. Chen, Richard J. Aguilar, Stephanie Weibel, Alandar A. Szalay
URN:urn:nbn:de:bvb:20-opus-119674
Document Type:Journal article
Faculties:Fakultät für Biologie / Rudolf-Virchow-Zentrum
Fakultät für Chemie und Pharmazie / Lehrstuhl für Biochemie
Language:English
Parent Title (English):PLoS ONE
ISSN:1932-6203
Year of Completion:2014
Volume:9
Issue:6
Pagenumber:e98533
Source:PLoS ONE 9(6): e98533. doi:10.1371/journal.pone.0098533
DOI:https://doi.org/10.1371/journal.pone.0098533
Pubmed Id:http://www.ncbi.nlm.nih.gov/pubmed?term=24887184
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 616 Krankheiten
Tag:cancer treatment; kidneys; lung and intrathoracic tumors; lymph nodes; metastasis; oncolytic viruses; renal cancer; secondary lung tumors
Release Date:2015/11/11
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung