The Proteome Profiles of the Cerebellum of Juvenile, Adult and Aged Rats-An Ontogenetic Study

Please always quote using this URN: urn:nbn:de:bvb:20-opus-151347
  • In this study, we searched for proteins that change their expression in the cerebellum (Ce) of rats during ontogenesis. This study focuses on the question of whether specific proteins exist which are differentially expressed with regard to postnatal stages of development. A better characterization of the microenvironment and its development may result from these study findings. A differential two-dimensional polyacrylamide gel electrophoresis (2DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS)In this study, we searched for proteins that change their expression in the cerebellum (Ce) of rats during ontogenesis. This study focuses on the question of whether specific proteins exist which are differentially expressed with regard to postnatal stages of development. A better characterization of the microenvironment and its development may result from these study findings. A differential two-dimensional polyacrylamide gel electrophoresis (2DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis of the samples revealed that the number of proteins of the functional classes differed depending on the developmental stages. Especially members of the functional classes of biosynthesis, regulatory proteins, chaperones and structural proteins show the highest differential expression within the analyzed stages of development. Therefore, members of these functional protein groups seem to be involved in the development and differentiation of the Ce within the analyzed development stages. In this study, changes in the expression of proteins in the Ce at different postnatal developmental stages (postnatal days (P) 7, 90, and 637) could be observed. At the same time, an identification of proteins which are involved in cell migration and differentiation was possible. Especially proteins involved in processes of the biosynthesis and regulation, the dynamic organization of the cytoskeleton as well as chaperones showed a high amount of differentially expressed proteins between the analyzed dates.show moreshow less

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Metadaten
Author: Michael Wille, Antje Schümann, Andreas Wree, Michael Kreutzer, Michael O. Glocker, Grit Mutzbauer, Oliver Schmitt
URN:urn:nbn:de:bvb:20-opus-151347
Document Type:Journal article
Faculties:Medizinische Fakultät / Pathologisches Institut
Language:English
Parent Title (English):International Journal of Molecular Sciences
Year of Completion:2015
Volume:16
Source:International Journal of Molecular Sciences 2015, 16, 21454-21485. DOI:10.3390/ijms160921454
DOI:https://doi.org/10.3390/ijms160921454
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Tag:adenosine kinase; binding; brain; cerebellum; coated vesicles; development; expression; hnRNP K; human brain; messenger RNA; neuronal differentiation; phosphatase 2A; postnatal development; proteins; proteomics; rat
Release Date:2017/10/10
First Page:21454
Last Page:21485
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International