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Differential expression of transposable elements in the medaka melanoma model
Please always quote using this URN: urn:nbn:de:bvb:20-opus-260615
- Malignant melanoma incidence is rising worldwide. Its treatment in an advanced state is difficult, and the prognosis of this severe disease is still very poor. One major source of these difficulties is the high rate of metastasis and increased genomic instability leading to a high mutation rate and the development of resistance against therapeutic approaches. Here we investigate as one source of genomic instability the contribution of activation of transposable elements (TEs) within the tumor. We used the well-established medaka melanoma modelMalignant melanoma incidence is rising worldwide. Its treatment in an advanced state is difficult, and the prognosis of this severe disease is still very poor. One major source of these difficulties is the high rate of metastasis and increased genomic instability leading to a high mutation rate and the development of resistance against therapeutic approaches. Here we investigate as one source of genomic instability the contribution of activation of transposable elements (TEs) within the tumor. We used the well-established medaka melanoma model and RNA-sequencing to investigate the differential expression of TEs in wildtype and transgenic fish carrying melanoma. We constructed a medaka-specific TE sequence library and identified TE sequences that were specifically upregulated in tumors. Validation by qRT- PCR confirmed a specific upregulation of a LINE and an LTR element in malignant melanomas of transgenic fish.…
Author: | Frederik HelmprobstORCiD, Susanne Kneitz, Barbara Klotz, Magali Naville, Corentin Dechaud, Jean-Nicolas Volff, Manfred SchartlORCiD |
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URN: | urn:nbn:de:bvb:20-opus-260615 |
Document Type: | Journal article |
Faculties: | Medizinische Fakultät / Theodor-Boveri-Institut für Biowissenschaften |
Language: | English |
Parent Title (English): | PLoS One |
Year of Completion: | 2021 |
Volume: | 16 |
Issue: | 10 |
Article Number: | e0251713 |
Source: | PLoS One (2021) 16:10, e0251713. https://doi.org/10.1371/journal.pone.0251713 |
DOI: | https://doi.org/10.1371/journal.pone.0251713 |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Tag: | cancer genomics; gene prediction; genomics; human genomics; malignant tumors; melanoma; retrotransposons; transposable elements |
Release Date: | 2022/03/31 |
Collections: | Open-Access-Publikationsfonds / Förderzeitraum 2021 |
Licence (German): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |