Structure Validation of G‐Rich RNAs in Noncoding Regions of the Human Genome
Please always quote using this URN: urn:nbn:de:bvb:20-opus-214892
- We present the rapid biophysical characterization of six previously reported putative G‐quadruplex‐forming RNAs from the 5′‐untranslated region (5′‐UTR) of silvestrol‐sensitive transcripts for investigation of their secondary structures. By NMR and CD spectroscopic analysis, we found that only a single sequence—[AGG]\(_{2}\)[CGG]\(_{2}\)C—folds into a single well‐defined G‐quadruplex structure. Sequences with longer poly‐G strands form unspecific aggregates, whereas CGG‐repeat‐containing sequences exhibit a temperature‐dependent equilibriumWe present the rapid biophysical characterization of six previously reported putative G‐quadruplex‐forming RNAs from the 5′‐untranslated region (5′‐UTR) of silvestrol‐sensitive transcripts for investigation of their secondary structures. By NMR and CD spectroscopic analysis, we found that only a single sequence—[AGG]\(_{2}\)[CGG]\(_{2}\)C—folds into a single well‐defined G‐quadruplex structure. Sequences with longer poly‐G strands form unspecific aggregates, whereas CGG‐repeat‐containing sequences exhibit a temperature‐dependent equilibrium between a hairpin and a G‐quadruplex structure. The applied experimental strategy is fast and provides robust readout for G‐quadruplex‐forming capacities of RNA oligomers.…
Author: | Oliver Binas, Irene Bessi, Harald Schwalbe |
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URN: | urn:nbn:de:bvb:20-opus-214892 |
Document Type: | Journal article |
Faculties: | Fakultät für Chemie und Pharmazie / Institut für Organische Chemie |
Language: | English |
Parent Title (English): | ChemBioChem |
Year of Completion: | 2020 |
Volume: | 21 |
Issue: | 11 |
First Page: | 1656 |
Last Page: | 1663 |
Source: | ChemBioChem 2020, 21(11):1656-1663. DOI: 10.1002/cbic.201900696 |
DOI: | https://doi.org/10.1002/cbic.201900696 |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 540 Chemie und zugeordnete Wissenschaften |
Tag: | G-quadruplexes; NMR spectroscopy; RNA; biophysical investigation; circular dichroism |
Release Date: | 2021/04/16 |
Licence (German): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |