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Recognition of the small regulatory RNA RydC by the bacterial Hfq protein
Zitieren Sie bitte immer diese URN: urn:nbn:de:bvb:20-opus-114191
- Bacterial small RNAs (sRNAs) are key elements of regulatory networks that modulate gene expression. The sRNA RydC of Salmonella sp. and Escherichia coli is an example of this class of riboregulators. Like many other sRNAs, RydC bears a 'seed' region that recognises specific transcripts through base-pairing, and its activities are facilitated by the RNA chaperone Hfq. The crystal structure of RydC in complex with E. coli Hfq at 3.48 angstrom resolution illuminates how the protein interacts with and presents the sRNA for target recognition.Bacterial small RNAs (sRNAs) are key elements of regulatory networks that modulate gene expression. The sRNA RydC of Salmonella sp. and Escherichia coli is an example of this class of riboregulators. Like many other sRNAs, RydC bears a 'seed' region that recognises specific transcripts through base-pairing, and its activities are facilitated by the RNA chaperone Hfq. The crystal structure of RydC in complex with E. coli Hfq at 3.48 angstrom resolution illuminates how the protein interacts with and presents the sRNA for target recognition. Consolidating the protein-RNA complex is a host of distributed interactions mediated by the natively unstructured termini of Hfq. Based on the structure and other data, we propose a model for a dynamic effector complex comprising Hfq, small RNA, and the cognate mRNA target.…
Autor(en): | Daniela Dimastrogiovanni, Kathrin S. Fröhlich, Katarzyna J. Bandyra, Heather A. Bruce, Susann Hohensee, Jörg Vogel, Ben F. Luisi |
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URN: | urn:nbn:de:bvb:20-opus-114191 |
Dokumentart: | Artikel / Aufsatz in einer Zeitschrift |
Institute der Universität: | Medizinische Fakultät / Institut für Molekulare Infektionsbiologie |
Sprache der Veröffentlichung: | Englisch |
Titel des übergeordneten Werkes / der Zeitschrift (Englisch): | eLife |
ISSN: | 2050-084X |
Erscheinungsjahr: | 2014 |
Band / Jahrgang: | 3 |
Heft / Ausgabe: | e05375 |
Originalveröffentlichung / Quelle: | eLife 2014; 3 : e05375. DOI: 10.7554/eLife.05375 |
DOI: | https://doi.org/10.7554/eLife.05375 |
Allgemeine fachliche Zuordnung (DDC-Klassifikation): | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Freie Schlagwort(e): | C-Terminus; Escherichia coli-Hfq; Hfq; SM-like protein; chaperone Hfq; crystal-structures; gene regulation; interaction surfaces; messenger-RNA; natively unstructured protein; noncoding RNAs; protein-RNA recognition; small RNA; soluble-RNAs; target recognition |
Datum der Freischaltung: | 26.06.2015 |
Lizenz (Deutsch): | CC BY: Creative-Commons-Lizenz: Namensnennung |