Tendon-derived stem cells from the long head of the biceps tendon
Please always quote using this URN: urn:nbn:de:bvb:20-opus-200370
- Objectives The long head of the biceps (LHB) is often resected in shoulder surgery and could therefore serve as a cell source for tissue engineering approaches in the shoulder. However, whether it represents a suitable cell source for regenerative approaches, both in the inflamed and non-inflamed states, remains unclear. In the present study, inflamed and native human LHBs were comparatively characterized for features of regeneration. Methods In total, 22 resected LHB tendons were classified into inflamed samples (n = 11) and non-inflamedObjectives The long head of the biceps (LHB) is often resected in shoulder surgery and could therefore serve as a cell source for tissue engineering approaches in the shoulder. However, whether it represents a suitable cell source for regenerative approaches, both in the inflamed and non-inflamed states, remains unclear. In the present study, inflamed and native human LHBs were comparatively characterized for features of regeneration. Methods In total, 22 resected LHB tendons were classified into inflamed samples (n = 11) and non-inflamed samples (n = 11). Proliferation potential and specific marker gene expression of primary LHB-derived cell cultures were analyzed. Multipotentiality, including osteogenic, adipogenic, chondrogenic, and tenogenic differentiation potential of both groups were compared under respective lineage-specific culture conditions. Results Inflammation does not seem to affect the proliferation rate of the isolated tendon-derived stem cells (TDSCs) and the tenogenic marker gene expression. Cells from both groups showed an equivalent osteogenic, adipogenic, chondrogenic and tenogenic differentiation potential in histology and real-time polymerase chain reaction (RT-PCR) analysis. Conclusion These results suggest that the LHB tendon might be a suitable cell source for regenerative approaches, both in inflamed and non-inflamed states. The LHB with and without tendinitis has been characterized as a novel source of TDSCs, which might facilitate treatment of degeneration and induction of regeneration in shoulder surgery.…
Author: | Jonas Schmalzl, Piet Plumhoff, Fabian Gilbert, Frank Gohlke, Christian Konrads, Ulrich Brunner, Franz Jakob, Regina Ebert, Andre F. Steinert |
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URN: | urn:nbn:de:bvb:20-opus-200370 |
Document Type: | Journal article |
Faculties: | Medizinische Fakultät / Lehrstuhl für Orthopädie |
Language: | English |
Parent Title (English): | Bone & Joint Research |
Year of Completion: | 2019 |
Volume: | 8 |
Issue: | 9 |
Pagenumber: | 414–424 |
Source: | Bone Joint Research 2019;8:414–424. doi: 10.1302/2046-3758.89.BJR-2018-0214.R2 |
DOI: | https://doi.org/10.1302/2046-3758.89.BJR-2018-0214.R2 |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Tag: | biceps tendon; mesenchymal stem cell; shoulder; tendon-derived stem cell; tissue engineering |
Release Date: | 2020/03/11 |
Collections: | Open-Access-Publikationsfonds / Förderzeitraum 2019 |
Licence (German): | CC BY-NC-ND: Creative-Commons-Lizenz: Namensnennung, Nicht kommerziell, Keine Bearbeitungen 4.0 International |