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Initial characterization of a Syap1 knock-out mouse and distribution of Syap1 in mouse brain and cultured motoneurons
Zitieren Sie bitte immer diese URN: urn:nbn:de:bvb:20-opus-187258
- Synapse-associated protein 1 (Syap1/BSTA) is the mammalian homologue of Sap47 (synapse-associated protein of 47 kDa) in Drosophila. Sap47 null mutant larvae show reduced short-term synaptic plasticity and a defect in associative behavioral plasticity. In cultured adipocytes, Syap1 functions as part of a complex that phosphorylates protein kinase B alpha/Akt1 (Akt1) at Ser\(^{473}\) and promotes differentiation. The role of Syap1 in the vertebrate nervous system is unknown. Here, we generated a Syap1 knock-out mouse and show that lack of Syap1Synapse-associated protein 1 (Syap1/BSTA) is the mammalian homologue of Sap47 (synapse-associated protein of 47 kDa) in Drosophila. Sap47 null mutant larvae show reduced short-term synaptic plasticity and a defect in associative behavioral plasticity. In cultured adipocytes, Syap1 functions as part of a complex that phosphorylates protein kinase B alpha/Akt1 (Akt1) at Ser\(^{473}\) and promotes differentiation. The role of Syap1 in the vertebrate nervous system is unknown. Here, we generated a Syap1 knock-out mouse and show that lack of Syap1 is compatible with viability and fertility. Adult knock-out mice show no overt defects in brain morphology. In wild-type brain, Syap1 is found widely distributed in synaptic neuropil, notably in regions rich in glutamatergic synapses, but also in perinuclear structures associated with the Golgi apparatus of specific groups of neuronal cell bodies. In cultured motoneurons, Syap1 is located in axons and growth cones and is enriched in a perinuclear region partially overlapping with Golgi markers. We studied in detail the influence of Syap1 knockdown and knockout on structure and development of these cells. Importantly, Syap1 knockout does not affect motoneuron survival or axon growth. Unexpectedly, neither knockdown nor knockout of Syap1 in cultured motoneurons is associated with reduced Ser\(^{473}\) or Thr\(^{308}\) phosphorylation of Akt. Our findings demonstrate a widespread expression of Syap1 in the mouse central nervous system with regionally specific distribution patterns as illustrated in particular for olfactory bulb, hippocampus, and cerebellum.…
Autor(en): | Dominique Schmitt, Natalia Funk, Robert Blum, Esther Asan, Lill Andersen, Thomas Rülicke, Michael Sendtner, Erich Buchner |
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URN: | urn:nbn:de:bvb:20-opus-187258 |
Dokumentart: | Artikel / Aufsatz in einer Zeitschrift |
Institute der Universität: | Medizinische Fakultät / Institut für Klinische Neurobiologie |
Medizinische Fakultät / Institut für Anatomie und Zellbiologie | |
Sprache der Veröffentlichung: | Englisch |
Titel des übergeordneten Werkes / der Zeitschrift (Englisch): | Histochemistry and Cell Biology |
Erscheinungsjahr: | 2016 |
Band / Jahrgang: | 146 |
Heft / Ausgabe: | 4 |
Seitenangabe: | 489-512 |
Originalveröffentlichung / Quelle: | Histochemistry and Cell Biology (2016) 146:4, 489-512. https://doi.org/10.1007/s00418-016-1457-0 |
DOI: | https://doi.org/10.1007/s00418-016-1457-0 |
Allgemeine fachliche Zuordnung (DDC-Klassifikation): | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Freie Schlagwort(e): | Axon growth; BSTA; Brain; Cells; Drosophilia; Glutamatergic synapses; Neurotrophic factors; PKB/Akt phosphorylation; Phosphorylation; Plasma-membrane; Protein kinase B; Rictor-mTOR complex; SAP47 gene; Spinal Muscular-arthropy; Syap1 localization; Viability |
Datum der Freischaltung: | 10.06.2020 |
EU-Projektnummer / Contract (GA) number: | 312325 |
OpenAIRE: | OpenAIRE |
Lizenz (Deutsch): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |