- search hit 1 of 1
Improving alginate printability for biofabrication: establishment of a universal and homogeneous pre-crosslinking technique
Please always quote using this URN: urn:nbn:de:bvb:20-opus-254030
- Many different biofabrication approaches as well as a variety of bioinks have been developed by researchers working in the field of tissue engineering. A main challenge for bioinks often remains the difficulty to achieve shape fidelity after printing. In order to overcome this issue, a homogeneous pre-crosslinking technique, which is universally applicable to all alginate-based materials, was developed. In this study, the Young’s Modulus after post-crosslinking of selected hydrogels, as well as the chemical characterization of alginate in termsMany different biofabrication approaches as well as a variety of bioinks have been developed by researchers working in the field of tissue engineering. A main challenge for bioinks often remains the difficulty to achieve shape fidelity after printing. In order to overcome this issue, a homogeneous pre-crosslinking technique, which is universally applicable to all alginate-based materials, was developed. In this study, the Young’s Modulus after post-crosslinking of selected hydrogels, as well as the chemical characterization of alginate in terms of M/G ratio and molecular weight, were determined. With our technique it was possible to markedly enhance the printability of a 2% (w/v) alginate solution, without using a higher polymer content, fillers or support structures. 3D porous scaffolds with a height of around 5 mm were printed. Furthermore, the rheological behavior of different pre-crosslinking degrees was studied. Shear forces on cells as well as the flow profile of the bioink inside the printing nozzle during the process were estimated. A high cell viability of printed NIH/3T3 cells embedded in the novel bioink of more than 85% over a time period of two weeks could be observed.…
Author: | Jonas Hazur, Rainer Detsch, Emine Karakaya, Joachim Kaschta, Jörg Teßmar, Dominik Schneidereit, Oliver Friedrich, Dirk W Schubert, Aldo R Boccaccini |
---|---|
URN: | urn:nbn:de:bvb:20-opus-254030 |
Document Type: | Journal article |
Faculties: | Medizinische Fakultät / Abteilung für Funktionswerkstoffe der Medizin und der Zahnheilkunde |
Language: | English |
Parent Title (English): | Biofabrication |
Year of Completion: | 2020 |
Volume: | 12 |
Issue: | 4 |
Article Number: | 045004 |
Source: | Biofabrication 2020, 12(4):045004. DOI: 10.1088/1758-5090/ab98e5 |
DOI: | https://doi.org/10.1088/1758-5090/ab98e5 |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Tag: | alginate; bioink; bioprinting; pre-crosslinking; printability; rheology; shape fidelity |
Release Date: | 2022/01/28 |
Licence (German): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |