5-Methylcytosine-Rich Heterochromatin in the Indian Muntjac
Please always quote using this URN: urn:nbn:de:bvb:20-opus-196701
- Two 5-methylcytosine (5-MeC)-rich heterochromatic regions were demonstrated in metaphase chromosomes of the Indian muntjac by indirect immunofluorescence using a monoclonal anti-5-MeC antibody. The metaphases were obtained from diploid and triploid cell lines. A major region is located in the ‘neck' of the 3;X fusion chromosome and can be detected after denaturation of the chromosomal DNA with UV-light irradiation for 1 h. It is located exactly at the border of the X chromosome and the translocated autosome 3. A minor region is found in theTwo 5-methylcytosine (5-MeC)-rich heterochromatic regions were demonstrated in metaphase chromosomes of the Indian muntjac by indirect immunofluorescence using a monoclonal anti-5-MeC antibody. The metaphases were obtained from diploid and triploid cell lines. A major region is located in the ‘neck' of the 3;X fusion chromosome and can be detected after denaturation of the chromosomal DNA with UV-light irradiation for 1 h. It is located exactly at the border of the X chromosome and the translocated autosome 3. A minor region is found in the centromeric region of the free autosome 3 after denaturing the chromosomal DNA for 3 h or longer. The structure and possible function of the major hypermethylated region as barrier against spreading of the X-inactivation process into the autosome 3 is discussed.…
Author: | Michael Schmid, Claus Steinlein, Christian Lomb, Karl Sperling, Heidemarie Neitzel |
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URN: | urn:nbn:de:bvb:20-opus-196701 |
Document Type: | Journal article |
Faculties: | Medizinische Fakultät / Institut für Humangenetik |
Language: | English |
Parent Title (English): | Cytogenetic and Genome Research |
ISSN: | 1424-8581 |
ISSN: | 1424-859X |
Year of Completion: | 2016 |
Volume: | 147 |
Issue: | 4 |
Pagenumber: | 240-246 |
Source: | Cytogenetic and Genome Research 2015;147(4):240–246. DOI: 10.1159/000444431 |
DOI: | https://doi.org/10.1159/000444431 |
Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/26959372 |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Tag: | 5-Methylcytosine; Indian muntjac; heterochromatin; immunofluorescence |
Release Date: | 2021/08/20 |
Date of first Publication: | 2016/03/10 |
Note: | This publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively. |
Licence (German): | Deutsches Urheberrecht |