Exploiting the Thermoresponsive Properties of Poly(2-oxazoline)s for Biofabrication
Anwendung der Thermoresponsivität von Poly(2-oxazolin) für die Biofabrikation
Please always quote using this URN: urn:nbn:de:bvb:20-opus-247462
- In this thesis, non-modified POx, namely PnPrOx and PcycloPrOx, with an LCST in the physiological range between 20 and 37°C have been utilized as materials for three different biofabrication approaches. Their thermoresponsive behavior and processability were exploited to establish an easy-to-apply coating for cell sheet engineering, a novel method to create biomimetic scaffolds based on aligned fibrils via Melt Electrowriting (MEW) and the application of melt electrowritten sacrificial scaffolds for microchannel creation for hydrogels.In this thesis, non-modified POx, namely PnPrOx and PcycloPrOx, with an LCST in the physiological range between 20 and 37°C have been utilized as materials for three different biofabrication approaches. Their thermoresponsive behavior and processability were exploited to establish an easy-to-apply coating for cell sheet engineering, a novel method to create biomimetic scaffolds based on aligned fibrils via Melt Electrowriting (MEW) and the application of melt electrowritten sacrificial scaffolds for microchannel creation for hydrogels. Chapter 3 describes the establishment of a thermoresponsive coating for tissue culture plates. Here, PnPrOx was simply dissolved in water and dried in well plates and petri dishes in an oven. PnPrOx adsorbed to the surface, and the addition of warm media generated a cell culture compatible coating. It was shown that different cell types were able to attach and proliferate. After confluency, temperature reduction led to the detachment of cell sheets. Compared to standard procedures for surface coating, the thermoresponsive polymer is not bound covalently to the surface and therefore does not require specialized equipment and chemical knowledge. However, it should be noted that the detachment of the cell layer requires the dissolution of the PnPrOx-coating, leading to possible polymer contamination. Although it is only a small amount of polymer dissolved in the media, the detached cell sheets need to be washed by media exchange for further processing if required. ...…
- In dieser Dissertation wurden die unmodifizierten Poly(2-oxazoline) PnPrOx und PcycloProx, welche eine LCST im physiologischen Bereich zwischen 20 und 37°C aufweisen, für drei verschiedene Biofabrikationsansätze verwendet. Deren Thermoresponsivität und Prozessierbarkeit wurde genutzt, um ein simples Beschichten von Oberflächen für Cell Sheet Engineering, eine neue Methode zur Herstellung biomimetischer Gerüststrukturen basierend auf der Generierung von Fibrillenbündeln via Melt Electrowriting und die Anwendung als Opferstrukturen zurIn dieser Dissertation wurden die unmodifizierten Poly(2-oxazoline) PnPrOx und PcycloProx, welche eine LCST im physiologischen Bereich zwischen 20 und 37°C aufweisen, für drei verschiedene Biofabrikationsansätze verwendet. Deren Thermoresponsivität und Prozessierbarkeit wurde genutzt, um ein simples Beschichten von Oberflächen für Cell Sheet Engineering, eine neue Methode zur Herstellung biomimetischer Gerüststrukturen basierend auf der Generierung von Fibrillenbündeln via Melt Electrowriting und die Anwendung als Opferstrukturen zur Generierung von Mikrokanälen in Hydrogelen zu etablieren.…
Author: | Matthias RymaORCiDGND |
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URN: | urn:nbn:de:bvb:20-opus-247462 |
Document Type: | Doctoral Thesis |
Granting Institution: | Universität Würzburg, Graduate Schools |
Faculties: | Graduate Schools / Graduate School of Life Sciences |
Medizinische Fakultät / Abteilung für Funktionswerkstoffe der Medizin und der Zahnheilkunde | |
Referee: | Prof. Dr. Jürgen Groll, Prof. Dr. Süleyman Ergün, Prof. Dr. Iwona Cicha |
Date of final exam: | 2021/10/14 |
Language: | English |
Year of Completion: | 2022 |
DOI: | https://doi.org/10.25972/OPUS-24746 |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 54 Chemie / 542 Techniken, Ausstattung, Materialien |
GND Keyword: | Thermoresponsive Polymere; Dihydrooxazole; 3D-Druck |
Tag: | Biofabrikation; Vaskularisation Cell Sheet Engineering; Melt Electrowriting; Poly(2-oxazoline) |
Release Date: | 2022/10/14 |
Licence (German): | Deutsches Urheberrecht mit Print on Demand |